Abstract
Transmission electron microscopy of pollen from Lilium longiflorum Thunb. reveals electron-dense inclusions in storage body organelles ubiquitous in the cytosol. In ungerminated pollen, these inclusions are rounded in appearance and appressed to the inner surface of the smooth membrane of the storage body. During pollen germination, these inclusions become less rounded, smaller, and enclosed in storage bodies that have developed crenated membranes. Energy dispersive x-ray analysis reveals high levels of P, Mg, K, and Ca in the inclusions relative to other regions of the cytosol in which elemental signals can be obtained. The elemental composition and the degradation of inclusions during germination are offered as evidence for storage of phytin in these structures which are thus analogous to phytin storage globoids of seed tissues.
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- LARSON D. A. FINE-STRUCTURAL CHANGES IN THE CYTOPLASM OF GERMINATING POLLEN. Am J Bot. 1965 Feb;52:139–154. [PubMed] [Google Scholar]
- Loewus F. A., Baldi B. G., Franceschi V. R., Meinert L. D., McCollum J. J. Pollen sporoplasts: dissolution of pollen walls. Plant Physiol. 1985 Jul;78(3):652–654. doi: 10.1104/pp.78.3.652. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lott J. N. Protein Body Composition in Cuburbita maxima Cotyledons as Determined by Energy Dispersive X-Ray Analysis. Plant Physiol. 1975 May;55(5):913–916. doi: 10.1104/pp.55.5.913. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lott J. N., Spitzer E., Vollmer C. M. Calcium distribution in globoid crystals of cucurbita cotyledon protein bodies. Plant Physiol. 1979 May;63(5):847–851. doi: 10.1104/pp.63.5.847. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sanger J. M., Jackson W. T. Fine structure study of pollen development in Haemanthus katherinae Baker. 3. Changes in organelles during development of the vegetative cell. J Cell Sci. 1971 Mar;8(2):317–329. doi: 10.1242/jcs.8.2.317. [DOI] [PubMed] [Google Scholar]
- Scott J. J., Loewus F. A. A Calcium-Activated Phytase from Pollen of Lilium longiflorum. Plant Physiol. 1986 Sep;82(1):333–335. doi: 10.1104/pp.82.1.333. [DOI] [PMC free article] [PubMed] [Google Scholar]