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. 1987 Jul;84(3):682–687. doi: 10.1104/pp.84.3.682

Biosynthesis of Sulfoquinovosyldiacylglycerol in Higher Plants

The Incorporation of 35SO4 by Intact Chloroplasts in Darkness

K F Kleppinger-Sparace 1,1, J B Mudd 1
PMCID: PMC1056650  PMID: 16665502

Abstract

Intact spinach chloroplasts incorporated 35SO42− into sulfoquinovosyldiacylglycerol in the dark at rates equivalent to those previously reported for illuminated chloroplasts provided that either ATP itself or an ATP-generating system was added. No additional reductant was necessary for SQDG synthesis by chloroplasts. The optimal concentration of ATP was between 2 and 3 millimolar. Rates of synthesis up to 2.6 nanomoles per milligram chlorophyll per hour were observed. UTP, GTP, and CTP could not substitute for ATP. Incubation of UTP with ATP (1:1) stimulated synthesis of sulfoquinovosyldiacylglycerol. No additional stimulation of the reaction was observed upon addition of other nucleoside triphosphates with ATP. For the generation of ATP in the chloroplast, addition of dihydroxyacetone phosphate alone did not promote synthesis of sulfoquinovosyldiacylglycerol, but in combination with inorganic phosphate and oxaloacetate, rates of synthesis up to 3.2 nanomoles per milligram chlorophyll per hour were observed. Dark synthesis was optimal in the presence of 2 millimolar dihydroxyacetone phosphate, 2 millimolar oxaloacetate, and 1 millimolar KH2PO4.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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