Abstract
Ethylene inducing proteins were partially purified and characterized from the cell wall digesting enzyme mixture, Cellulysin. Purification included binding to Sephacryl S-200, isoelectric focusing, molecular sieving on Sephadex G-75, agarose electrophoresis, and sizing using a Superose 12 column. At least three active proteins were obtained from the Sephadex G-75 fraction that move towards the cathode during nondenaturing agarose electrophoresis. These three protein fractions separated by preparative agarose electrophoresis contain polypeptide patterns that are very similar on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The fractions contain three main Coomassie blue stained bands of about 10, 14, and 18 kilodaltons. Gel filtration of the major fraction on a Superose 12 column yields an active peak with an apparent molecular weight of 27,000. Proteolytic enzymes, in the presence of urea, destroy the ethylene inducing activity. We conclude that the ethylene inducing factor (EIF) that we have isolated from Cellulysin is protein. Similar ethylene inducing factors are present in Cellulase RS. Ethylene inducing components from pectinase, Pectolyase, and Rhozyme do not bind to Sephacryl like EIF from Cellulysin. Thus, the components responsible for the ethylene inducing activity in these latter enzyme preparations differ from that of EIF.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Anderson J. D., Lieberman M., Stewart R. N. Ethylene production by apple protoplasts. Plant Physiol. 1979 May;63(5):931–935. doi: 10.1104/pp.63.5.931. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Anderson J. D., Mattoo A. K., Lieberman M. Induction of ethylene biosynthesis in tobacco leaf discs by cell wall disesting enzymes. Biochem Biophys Res Commun. 1982 Jul 30;107(2):588–596. doi: 10.1016/0006-291x(82)91532-7. [DOI] [PubMed] [Google Scholar]
- Baker J. E. Superoxide dismutase in ripening fruits. Plant Physiol. 1976 Nov;58(5):644–647. doi: 10.1104/pp.58.5.644. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Chalutz E., Mattoo A. K., Solomos T., Anderson J. D. Enhancement by ethylene of cellulysin-induced ethylene production by tobacco leaf discs. Plant Physiol. 1984 Jan;74(1):99–103. doi: 10.1104/pp.74.1.99. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tong C. B., Labavitch J. M., Yang S. F. The induction of ethylene production from pear cell culture by cell wall fragments. Plant Physiol. 1986 Jul;81(3):929–930. doi: 10.1104/pp.81.3.929. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Zacharius R. M., Zell T. E., Morrison J. H., Woodlock J. J. Glycoprotein staining following electrophoresis on acrylamide gels. Anal Biochem. 1969 Jul;30(1):148–152. doi: 10.1016/0003-2697(69)90383-2. [DOI] [PubMed] [Google Scholar]