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. 1987 Jul;84(3):918–923. doi: 10.1104/pp.84.3.918

Temperature-Induced Protein Conformational Changes in Barley Root Plasma Membrane-Enriched Microsomes

I. Effect of Temperature on Membrane Protein and Lipid Mobility

Charles R Caldwell 1, Carol E Whitman 1
PMCID: PMC1056695  PMID: 16665544

Abstract

A protein spin label and lipid spin probes were used to study the temperature-dependent motion of protein and lipid, respectively, in barley (Hordeum vulgare L. cv Conquest) root plasma membrane-enriched microsomes. Using membranes from seedlings grown at 20°C, the temperature-dependence of the relative motion of membrane surface spin probes and a spin label covalently attached to membrane proteins suggested abrupt changes in the lipid and protein mobilities at about 12°C. Spin probe spin-spin exchange broadening and fluorescent probe eximer formation indicated apparent temperature-induced alterations in probe lateral diffusion within the membrane at about 12 to 14°C. The results suggest the presence of temperature-induced quasicrystalline lipid clusters which may influence the activity of membrane-bound enzymes.

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Selected References

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