Skip to main content
PMC home page
Plant Physiology logoLink to Plant Physiology
. 1987 Aug;84(4):1451–1456. doi: 10.1104/pp.84.4.1451

Photosynthetic Characteristics of a Photoautotrophic Cell Suspension Culture of Soybean 1

Suzanne M D Rogers 1, William L Ogren 1, Jack M Widholm 1
PMCID: PMC1056795  PMID: 16665626

Abstract

A soybean suspension culture (SB-P) which can grow photoautotrophically in 5% CO2 will not grow in ambient CO2 levels. This elevated CO2 requirement seems to be due to the additive effects of a number of factors. The in vivo activity of ribulose-1,5-bisphosphate carboxylase (RuBPcase) is much lower in the SB-P cells, compared to soybean plants. This may be due to the low light intensity used to culture the cells, which has been shown to decrease both the amount and activity in whole plants, resulting in a low rate of net photosynthesis. The RuBPcase activation level is also lowered in air CO2 levels. The presence of the liquid medium raises the cells CO2 compensation concentration (the CO2 concentration reached when the rates of CO2 fixed by photosynthesis and the CO2 respired by the cells are equal). These factors, coupled with the high respiratory loss of CO2 all contribute to reduced net photosynthesis in air, resulting in a photosynthetic capacity that is inadequate for cell survival. Active cell division, low photosynthetic capacity, elevated respiration, and a low ratio of RuBPcase(initial)/phosphoenolpyruvate carboxylase are traits that SB-P cells share with young leaf cells, indicating SB-P cell physiology may be comparable to that of young expanding leaves rather than to that of mature leaves.

Full text

PDF
1451

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arnon D. I. COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS. Plant Physiol. 1949 Jan;24(1):1–15. doi: 10.1104/pp.24.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Berlyn M. B., Zelitch I., Beaudette P. D. Photosynthetic characteristics of photoautotrophically grown tobacco callus cells. Plant Physiol. 1978 Apr;61(4):606–610. doi: 10.1104/pp.61.4.606. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Horn M. E., Sherrard J. H., Widholm J. M. Photoautotrophic growth of soybean cells in suspension culture: I. Establishment of photoautotrophic cultures. Plant Physiol. 1983 Jun;72(2):426–429. doi: 10.1104/pp.72.2.426. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Quebedeaux B., Chollet R. Growth and Development of Soybean (Glycine max [L.] Merr.) Pods: CO(2) Exchange and Enzyme Studies. Plant Physiol. 1975 Apr;55(4):745–748. doi: 10.1104/pp.55.4.745. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Salvucci M. E., Portis A. R., Ogren W. L. Light and CO(2) Response of Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase Activation in Arabidopsis Leaves. Plant Physiol. 1986 Mar;80(3):655–659. doi: 10.1104/pp.80.3.655. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Seeni S., Gnanam A. Carbon Assimilation in Photoheterotrophic Cells of Peanut (Arachis hypogaea L.) Grown in Still Nutrient Medium. Plant Physiol. 1982 Sep;70(3):823–826. doi: 10.1104/pp.70.3.823. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Servaites J. C. Chemical inhibition of the glycolate pathway in soybean leaf cells. Plant Physiol. 1977 Oct;60(4):461–466. doi: 10.1104/pp.60.4.461. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Servaites J. C., Torisky R. S. Activation state of ribulose bisphosphate carboxylase in soybean leaves. Plant Physiol. 1984 Mar;74(3):681–686. doi: 10.1104/pp.74.3.681. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Smith E. W., Tolbert N. E., Ku H. S. Variables Affecting the CO(2) Compensation Point. Plant Physiol. 1976 Aug;58(2):143–146. doi: 10.1104/pp.58.2.143. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Spector T. Refinement of the coomassie blue method of protein quantitation. A simple and linear spectrophotometric assay for less than or equal to 0.5 to 50 microgram of protein. Anal Biochem. 1978 May;86(1):142–146. doi: 10.1016/0003-2697(78)90327-5. [DOI] [PubMed] [Google Scholar]
  11. Thornley J. H. Respiration, growth and maintenance in plants. Nature. 1970 Jul 18;227(5255):304–305. doi: 10.1038/227304b0. [DOI] [PubMed] [Google Scholar]
  12. Vu C. V., Allen L. H., Bowes G. Effects of Light and Elevated Atmospheric CO(2) on the Ribulose Bisphosphate Carboxylase Activity and Ribulose Bisphosphate Level of Soybean Leaves. Plant Physiol. 1983 Nov;73(3):729–734. doi: 10.1104/pp.73.3.729. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES