Psychological and Cognitive Sciences Correction for “GABAergic miR-34a regulates Dorsal Raphè inhibitory transmission in response to aversive, but not rewarding, stimuli,” by Donald Ielpo, Serafina M. Guzzo, Giovanni F. Porcheddu, Maria Teresa Viscomi, Clarissa Catale, Ingrid Reverte, Simona Cabib, Carlo Cifani, Gabriella Antonucci, Rossella Ventura, Luisa Lo Iacono, Cristina Marchetti, and Diego Andolina which published July 31, 2023; 10.1073/pnas.2301730120 (Proc. Natl. Acad. Sci. U.S.A. 120, e2301730120).
The authors note that Fig. 3G appeared incorrectly: The scatterplots for “Neg. Ctrl—Stress” and “Neg. Ctrl—Chocolate” should not be identical. The corrected figure and its legend appear below. The online version has been corrected.
Fig. 3.
Inhibition of miR-34a in the DRN through an antagomiR-34a (Ant-34a) potentiates local GABA release in response to an aversive (restraint stress), but not a rewarding (white chocolate), stimulus. Experimental timelines: (A) Two days after the stereotaxic surgery, Ant-34a, or negative control (Neg. Ctrl.) were administered. Immediately after the administration, the 2-h restraint stress procedure started. (B) In case of the rewarding stimulus protocol, Ant-34a or Neg. Ctrl. was administered 2 d before exposure to white chocolate, to avoid immobilization-induced stress. (C) In vivo microdialysis data show that intra-DRN infusion of Ant-34a in C57BL/6 mice exposed to 120 min of restraint stress (N = 7) caused a time-dependent increase of GABA outflow compared to the Neg. Ctrl.-treated group (N = 8), *P < 0.05 in comparison with the corresponding time point of the Neg. Ctrl. group. (D) Conversely, 120 min of white chocolate exposure significantly reduced GABA outflow regardless of the Ant-34a/Neg.Ctrl. treatment (Ant-34a group N = 6; Neg.Ctrl. Group N = 9), *P < 0.05, from basal values (Neg. Ctrl. -treated group). #P < 0.05, ##P < 0.01 from basal values (Ant-34a-treated group). Microdialysis results are expressed as percent changes (means ± SEM) from basal values. (E) Representative traces of mIPSCs. (F and G) mean ± SEM of mIPSC frequency and amplitude recorded from 5-HT neurons in the absence of any stimulus, at the end of the restraint protocol or of the exposure to chocolate, in animals infused with Ant-34a or Neg. Ctrl., *P < 0.05, **P < 0.01, ns: non-significant. Number of cells per number of animals (n/N) are indicated below each group in panel F.