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. 1961 Nov;9(6):484–486. doi: 10.1128/am.9.6.484-486.1961

Isolation of Salmonellae from Foods Samples

IV. Comparison of Methods of Enrichment

Welton I Taylor 1,1, John H Silliker 1
PMCID: PMC1057771  PMID: 13920002

Abstract

A comparison of various methods of enhancing frequency of Salmonella isolations revealed that inoculation of a second enrichment broth, with culture from the first, was no improvement over the single direct enrichment method. It was inferior to centrifugation.

Selenite was observed to produce more positive isolations at 48 hr than at 24. No change occurred in tetrathionate. Reconstitution of dried albumen with water produced a significant increase in isolations over direct inoculation of enrichment broth in the case of tetrathionate but not selenite broth.

Pre-enrichment in lactose broth before inoculation of enrichment media was vastly superior to reconstitution in water for both enrichment broths. A comparison of results obtained using dulcitol, mannitol, lactose and carbohydrate-free purple broths in pre-enrichment indicated that the carbohydrate added was immaterial.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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