FIGURE 2.

Design and activity of 50S subunits with MS2 tags inserted into H98. (A) The secondary structure of H98 in WT 23S rRNA or ribosomes with MS2 tags. The sequence of MS2-V1 and MS2-V2 are from Youngman and Green (2005) and Ward et al. (2019), respectively. Addition of an adenosine in MS2-V3 at a position equivalent to A2800 is proposed to mimic the sequence of the WT 23S rRNA (A2800 in red). 50S subunits were preincubated at (B) 37°C or (C) 60°C before their addition to the nanoLuciferase in vitro translation assay. Data are normalized to WT subunits with no MS2 tag. All error bars are the standard deviation of three experimental replicates.