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. 1962 Nov;10(6):577–579. doi: 10.1128/am.10.6.577-579.1962

Ballistic Disruption of Penicillium chrysogenum Cells

Pat B Hamilton 1,1, S G Knight 1
PMCID: PMC1057918  PMID: 13952345

Abstract

The use of glass beads in a high-speed mixing device to rupture organisms was applied to molds. The use of a mixer in which the propellor shaft enters from the top into a metal mixing chamber made it possible to immerse the whole device in a salt water and ice mixture so that the temperature of the glass-bead slurry could be kept below 5 C without difficulty. Mycelia, glass beads, and buffer in a 1:2:3 (w/w) ratio gave above 95% breakage in 15 min with Penicillium chrysogenum cells and in 4 min with Rhizopus nigricans. Some of the factors influencing breakage are discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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