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. 1962 Nov;10(6):580–582. doi: 10.1128/am.10.6.580-582.1962

Continuous Culture of Ruminal Microorganisms in Chemically Defined Medium1

I. Design of Continuous-Culture Apparatus

Loyd Y Quinn 1
PMCID: PMC1057919  PMID: 13972781

Abstract

An apparatus is described which has been used for successful continuous culture of the ciliates from the rumen of cattle. Automatic control of feeding rate, pH, oxidation-reduction potential, temperature, stirring rate, aeration rate, salinity, and volume of the culture is provided for, using standard commercial equipment, whenever possible. The operation of this apparatus is described.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ADLER J. H., DYE J. A., BOGGS D. E., WILLIAMS H. H. Growth of rumen microorganisms in an in vitro continuous-flow system on a protein-free diet. Cornell Vet. 1958 Jan;48(1):53–66. [PubMed] [Google Scholar]
  2. COLEMAN G. S. Maintenance of oligotrich protozoa from the sheep rumen in vitro. Nature. 1958 Oct 18;182(4642):1104–1105. doi: 10.1038/1821104a0. [DOI] [PubMed] [Google Scholar]
  3. COLEMAN G. S. The cultivation of sheep rumen oligotrich protozoa in vitro. J Gen Microbiol. 1960 Apr;22:555–563. doi: 10.1099/00221287-22-2-555. [DOI] [PubMed] [Google Scholar]
  4. GUTIERREZ J. Experiments on the culture and physiology of holotriches from the bovine rumen. Biochem J. 1955 Jul;60(3):516–522. doi: 10.1042/bj0600516. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. HEALD P. J., OXFORD A. E. Fermentation of soluble sugars by anaerobic holotrich ciliate protozoa of the genera Isotricha and Dasytricha. Biochem J. 1953 Feb;53(3):506–512. doi: 10.1042/bj0530506. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. HUNGATE R. E. Symposium: selected topics in microbial ecology. I. Microbial ecology of the rumen. Bacteriol Rev. 1960 Dec;24(4):353–364. doi: 10.1128/br.24.4.353-364.1960. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hastings E. G. THE SIGNIFICANCE OF THE BACTERIA AND THE PROTOZOA OF THE RUMEN OF THE BOVINE . Bacteriol Rev. 1944 Dec;8(4):235–254. doi: 10.1128/br.8.4.235-254.1944. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. LOUW J. G., WILLIAMS H. H., MAYNARD L. A. A new method for the study in vitro of rumen digestion. Science. 1949 Nov 4;110(2862):478–480. doi: 10.1126/science.110.2862.478. [DOI] [PubMed] [Google Scholar]
  9. MCKEE M. T. Bacterial culture with controlled pH. Appl Microbiol. 1955 Nov;3(6):355–360. doi: 10.1128/am.3.6.355-360.1955. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. OXFORD A. E. The rumen ciliate protozoa: their chemical composition, metabolism, requirements for maintenance and culture, and physiological significance for the host. Exp Parasitol. 1955 Nov;4(6):569–605. doi: 10.1016/0014-4894(55)90045-x. [DOI] [PubMed] [Google Scholar]
  11. QUINN L. Y., BURROUGHS W., CHRISTIANSEN W. C. Continuous culture of ruminal microorganisms in chemically defined medium. II. Culture medium studies. Appl Microbiol. 1962 Nov;10:583–592. doi: 10.1128/am.10.6.583-592.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. SUGDEN B., OXFORD A. E. Some cultural studies with holotrich ciliate protozoa of the sheep's rumen. J Gen Microbiol. 1952 Aug;7(1-2):145–153. doi: 10.1099/00221287-7-1-2-145. [DOI] [PubMed] [Google Scholar]
  13. SUGDEN B. The cultivation and metabolism of oligotrich Protozoa from the sheep's rumen. J Gen Microbiol. 1953 Aug;9(1):44–53. doi: 10.1099/00221287-9-1-44. [DOI] [PubMed] [Google Scholar]
  14. Stewart D. G., Warner R. G., Seeley H. W. Continuous Culture as a Method for Studying Rumen Fermentation. Appl Microbiol. 1961 Mar;9(2):150–156. doi: 10.1128/am.9.2.150-156.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. WARNER A. C. Criteria for establishing the validity of in vitro studies with rumen micro-organisms in so-called artificial rumen systems. J Gen Microbiol. 1956 Jul;14(3):733–748. doi: 10.1099/00221287-14-3-733. [DOI] [PubMed] [Google Scholar]

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