Zhou J, Wu Z, Zhang Z, et al. Pan-ERBB kinase inhibition augments CDK4/6 inhibitor efficacy in oesophageal squamous cell carcinoma. Gut 2022;71:665–75. doi:10.1136/gutjnl-2020-323276
Following publication of the original article, authors identified an error in figure 3B, specifically: TE9 cell line total HER3, total AKT and total ERK lanes appear to be misused. The correct figure is presented below. This correction does not affect the result and conclusions of the study. We apologise for this error and any inconvenience caused.
Figure 3 Pan ERBB and CDK4/6 pathway dual inhibition demonstrated efficacy in ESCC. (A) Images showing crystal violet staining of representative squamous carcinoma cell lines on treatment with afatinib (20 nM), palbociclib (500 nM), the combination or with DMSO control for 7–10 days. Data from one representative experiment are presented (n=2). (B) Immunoblot analysis of genes involved in ERBB signalling pathway and cell-cycle pathway in TE9, TE11 and KYSE180 cells treated with afatinib (20 nM), palbociclib (500 nM), the combination or with DMSO control. Protein lysates were collected after drug treatment for 24 hours, 48 hours and 72 hours. Immunoblots from one representative experiment (n=2) are shown. (C) The frequency of G0/G1 cells of squamous carcinoma cell lines on treatment with afatinib (20 nM), palbociclib (500 nM), the combination or with DMSO control for 24 hours (top) and 48 hours (bottom). Following treatment, the cells were harvested, stained with propidium iodide and assayed with flow cytometry. Data are shown as mean±SD and ns: not significant, *p<0.05, **p<0.01, ***p<0.001 and ****p<0.0001 as calculated by the two-way ANOVA test followed by post-hoc test with Benjamini-Hochberg correction. (D) Heatmap showing the expression of common E2F target genes in KYSE180 on treatment of DMSO, 20 nM afatinib, 500 nM palbocilib or combination. (E) Top: growth curve for KYS410 xenograft tumours (n=6–10) treated with vehicle, afatinib (5 mg/kg), palbociclib (50 mg/kg) or the combination. Data are shown as mean±SEM and ****p<0.0001 as calculated by the two-way ANOVA test followed by post-hoc test with Benjamini-Hochberg correction on day 31. Bottom: waterfall plot showing the tumour volume change (at day31) relative to baseline volume (at day1). Each bar represents one xenograft tumour. (F) Top: growth curve for TE11 xenograft tumours (n=6–10) treated with vehicle, afatinib (5 mg/kg), palbociclib (50 mg/kg) or the combination. Data are shown as mean±SEM and **p<0.01, ***p<0.001 and ****p<0.0001 as calculated by the two-way ANOVA test followed by post-hoc test with Benjamini-Hochberg correction on day 31. Bottom: waterfall plot showing the tumour volume change (at day 31) relative to baseline volume (at day1). Each bar represents one xenograft tumour.