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. 1963 Mar;11(2):122–127. doi: 10.1128/am.11.2.122-127.1963

Cephalothin, a New Cephalosporin with a Broad Antibacterial Spectrum

I. In Vitro Studies Employing the Gradient Plate Technique

C W Godzeski 1, Gordon Brier 1, D E Pavey 1
PMCID: PMC1057954  PMID: 13963283

Abstract

Cephalothin is 7-(thiophene-2-acetamido) cephalosporanic acid; it was prepared by N-acylation of the nucleus of cephalosporin C, 7-aminocephalosporanic acid. Cephalothin had a broad spectrum of antibiotic activity that was essentially unaffected by human serum or inoculum level, the activity of penicillinase, or pH variation of the growth medium. In vitro development of resistance by staphylococci could not be demonstrated, but the gram-negative organisms did develop a stepwise type of resistance to the antibiotic. Staphylococci made resistant in vitro to 5-methyl-3-phenyl-4-isoxazole penicillin were also resistant to cephalothin and to 6-(2,6-dimethoxybenzamido) penicillin; however, the mechanism of resistance to each antibiotic may have differed. Some complications involved in the laboratory evaluation methods currently in use in the field of antibiotics are examined.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ABRAHAM E. P., NEWTON G. G. A comparison of the action of penicillinase on benzylpenicillin and cephalosporin N and the competitive inhibition of penicillinase by cephalosporin C. Biochem J. 1956 Aug;63(4):628–634. doi: 10.1042/bj0630628. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. ABRAHAM E. P., NEWTON G. G. The structure of cephalesporin C. Biochem J. 1961 May;79:377–393. doi: 10.1042/bj0790377. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. BARBER M., WATERWORTH P. M. Antibacterial activity of the penicillins. Br Med J. 1962 Apr 28;1(5286):1159–1164. doi: 10.1136/bmj.1.5286.1159. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. BATCHELOR F. R., DOYLE F. P., NAYLER J. H., ROLINSON G. N. Synthesis of penicillin: 6-aminopenicillanic acid in penicillin fermentations. Nature. 1959 Jan 24;183(4656):257–258. doi: 10.1038/183257b0. [DOI] [PubMed] [Google Scholar]
  5. BUNN P. A., AMBERG J. Initial clinical and laboratory experiences with methyl phenyl isoxazolyl penicillin (P-12). N Y State J Med. 1961 Dec 15;61:4158–4162. [PubMed] [Google Scholar]
  6. Bryson V., Szybalski W. Microbial Selection. Science. 1952 Jul 18;116(3003):45–51. doi: 10.1126/science.116.3003.45. [DOI] [PubMed] [Google Scholar]
  7. GOUREVITCH A., HUNT G. A., PURSIANO T. A., CARMACK C. C., MOSES A. J., LEIN J. Microbiological evaluation of 5-methyl-3-phenyl-4-isoxazolyl penicillin. Antibiot Chemother (Northfield) 1961 Dec;11:780–789. [PubMed] [Google Scholar]
  8. KAGAN B. M., MOLANDER C. W., WEINBERGER H. J. Induction and cultivation of staphylococcal L forms in the presence of methicillin. J Bacteriol. 1962 May;83:1162–1163. doi: 10.1128/jb.83.5.1162-1163.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. LODER B., NEWTON G. G., ABRAHAM E. P. The cephalosporin C nucleus (7-aminocephalosporanic acid) and some of its derivatives. Biochem J. 1961 May;79:408–416. doi: 10.1042/bj0790408. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. NEWTON G. G., ABRAHAM E. P. Isolation of cephalosporin C, a penicillin-like antibiotic containing D-alpha-aminoadipic acid. Biochem J. 1956 Apr;62(4):651–658. doi: 10.1042/bj0620651. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. ROLINSON G. N., STEVENS S., BATCHELOR F. R., WOOD J. C., CHAIN E. B. Bacteriological studies on a new penicillin-BRL. 1241. Lancet. 1960 Sep 10;2(7150):564–567. doi: 10.1016/s0140-6736(60)91642-1. [DOI] [PubMed] [Google Scholar]

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