Abstract
A bacterial strain (NRRL B-1973) isolated from soil at Guatemala City and tentatively identified as an Arthrobacter species produced a polysaccharide with unusual properties. Conditions were studied for the production of this microbial gum in shaken flasks and 20-liter fermentors. Suitable nutrients for optimal polysaccharide production included 3% glucose, 0.3% enzyme-hydrolyzed casein, magnesium sulfate, manganese sulfate, and potassium phosphate buffer (pH 7.0). Polysaccharide yields ranged from 40 to 45%, based on initial dextrose in the medium in 3- or 4-day fermentations. The gum was readily recovered from culture fluid by alcohol precipitation in the presence of an electrolyte. The Arthrobacter gum exhibited characteristics unique for a polyelectrolyte. Viscosity of solutions was not decreased by heating in the presence of salt, and the gum withstood a temperature of 121 C for 30 min. At polysaccharide levels above 0.75%, gels were formed when solutions were autoclaved with KCl. There was no significant change in viscosity over a pH range of 5.0 to 10.0.
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- ANDERSON R. F., CADMUS M. C., BENEDICT R. G., SLODKI M. E. Laboratory production of a phosphorylated mannan by Hansenula holstii. Arch Biochem Biophys. 1960 Aug;89:289–292. doi: 10.1016/0003-9861(60)90056-4. [DOI] [PubMed] [Google Scholar]
- CADMUS M. C., LAGODA A. A., ANDERSON R. F. Production of a new polysaccharide with Cryptococcus laurentii var. flavescens. Appl Microbiol. 1962 Mar;10:153–156. doi: 10.1128/am.10.2.153-156.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
- CORMAN J., TSUCHIYA H. M., KOEPSELL H. J., BENEDICT R. G., KELLEY S. E., FEGER V. H., DWORSCHACK R. G., JACKSON R. W. Oxygen absorption rates in laboratory and pilot plant equipment. Appl Microbiol. 1957 Sep;5(5):313–318. doi: 10.1128/am.5.5.313-318.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HAYNES W. C., WICKERHAM L. J., HESSELTINE C. W. Maintenance of cultures of industrially important microorganisms. Appl Microbiol. 1955 Nov;3(6):361–368. doi: 10.1128/am.3.6.361-368.1955. [DOI] [PMC free article] [PubMed] [Google Scholar]