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. Author manuscript; available in PMC: 2023 Oct 17.
Published in final edited form as: Allergy. 2021 May 31;77(1):207–217. doi: 10.1111/all.14898

Figure 5. STAT3 represses CLEC7A through CENSER.

Figure 5.

(a) representative flow plots and (b) frequencies of STAT3 pTyr705 phosphoflow in CRSwNP hSNECs treated with IL-33 for 2h. CLEC7A promoter activity in (c) bronchial epithelial cell line or (d) Clec7a mRNA in isolated mouse tracheal epithelial cells from Stat3+/+ and Stat3−/− mice. (e) STAT3 binding at the CLEC7A locus in bronchial epithelial cell line treated with media or IL-33 (10 ng/ml) for 2h. CLEC7A mRNA in CRSwNP SNECs treated with (f) HDM (100 ug/ml) or (g) IL-33 (0.25 ng/ml) and vehicle or 5 uM STAT3 inhibitor, stattic for 2h. Data is means+SEM and representative of 2–3 independent experiments, representative of 3 donors (a,b,f,g). Data was analyzed by one-way ANOVA followed by post-hoc test or two-way ANOVA followed by Bonferroni’s multiple comparisons test (panel e, where significances are displayed for IL-33 (anti-STAT3) vs. IL-33 (anti-GFP)). *p<0.05; **p<0.01; ***p<0.001, ****p<0.0001.