Abstract
Alkyl sulfatase was induced by growth on nutrient broth plus sodium dodecyl sulfate (SDS) in a bacterium we have designated Pseudomonas C12B. Measurement of the radioactivity of S35O4= released from SDS35 by the enzyme in cell-free extracts provided an effective assay technique. The barium chloranilate assay for release of SO4= from SDS was somewhat less sensitive but effective if analyzed at 332 mμ. This test was only approximately 55% as sensitive if analyzed at 530 mμ. The activity of the glyoxylate bypass enzymes, isocitrate lyase and malate synthetase, was significantly stimulated by growth of the bacteria on SDS as the sole carbon source, but not by growth on nutrient broth or nutrient broth plus SDS.
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Selected References
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- HSU Y. C. DETERGENT (SODIUM LAURYL SULPHATE)-SPLITTING ENZYME FROM BACTERIA. Nature. 1963 Dec 14;200:1091–1092. doi: 10.1038/2001091b0. [DOI] [PubMed] [Google Scholar]
- PAYNE W. J., FEISAL V. E. Bacterial utilization of dodecyl sulfate and dodecyl benzene sulfonate. Appl Microbiol. 1963 Jul;11:339–344. doi: 10.1128/am.11.4.339-344.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
- RAMMLER D. H., GRADO C., FOWLER L. R. SULFUR METABOLISM OF AEROBACTER AEROGENES. I. A REPRESSIBLE SULFATASE. Biochemistry. 1964 Feb;3:224–230. doi: 10.1021/bi00890a014. [DOI] [PubMed] [Google Scholar]