Abstract
Immunization of rabbits with approximately 95% pure enterotoxin B and approximately 20 and 50% pure enterotoxin A yielded specific antisera which required no and little absorption, respectively, when used in the slide double-diffusion test. Methods for purifying enterotoxin A and for the production and absorption of antisera are presented.
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- Achorn G. B., Jr, Schwab J. L. A Method for the Aeration of Liquid Cultures of Microorganisms. Science. 1948 Apr 9;107(2780):377–378. doi: 10.1126/science.107.2780.377-a. [DOI] [PubMed] [Google Scholar]
- BERGDOLL M. S., SUGIYAMA H., DACK G. M. Staphylococcal enterotoxin. I. Purification. Arch Biochem Biophys. 1959 Nov;85:62–69. doi: 10.1016/0003-9861(59)90447-3. [DOI] [PubMed] [Google Scholar]
- CASMAN E. P., BENNETT R. W. CULTURE MEDIUM FOR THE PRODUCTION OF STAPHYLOCOCCAL ENTEROTOXIN A. J Bacteriol. 1963 Jul;86:18–23. doi: 10.1128/jb.86.1.18-23.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
- CASMAN E. P., BERGDOLL M. S., ROBINSON J. DESIGNATION OF STAPHYLOCOCCAL EXTEROTOXINS. J Bacteriol. 1963 Mar;85:715–716. doi: 10.1128/jb.85.3.715-716.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
- CASMAN E. P. Further serological studies of staphylococcal enterotoxin. J Bacteriol. 1960 Jun;79:849–856. doi: 10.1128/jb.79.6.849-856.1960. [DOI] [PMC free article] [PubMed] [Google Scholar]
- CASMAN E. P. Serologic studies of staphylococcal enterotoxin. Public Health Rep. 1958 Jul;73(7):599–609. [PMC free article] [PubMed] [Google Scholar]
- CROWLE A. J. A simplified micro double-diffusion agar precipitin technique. J Lab Clin Med. 1958 Nov;52(5):784–787. [PubMed] [Google Scholar]
- GORDON M., FIOCK M. A., YARINSKY A., DUFF J. T. Studies on immunity to toxins of Clostridium botulinum. III. Preparation, purification, and detoxification of type E toxin. J Bacteriol. 1957 Oct;74(4):533–538. doi: 10.1128/jb.74.4.533-538.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
- OUCHTERLONY O. Antigen-antibody reactions in gels. IV. Types of reactions in coordinated systems of diffusion. Acta Pathol Microbiol Scand. 1953;32(2):230–240. [PubMed] [Google Scholar]
- WADSWORTH C. A slide microtechnique for the analysis of immune precipitates in gel. Int Arch Allergy Appl Immunol. 1957;10(6):355–360. doi: 10.1159/000228394. [DOI] [PubMed] [Google Scholar]