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. 1964 Sep;12(5):436–441. doi: 10.1128/am.12.5.436-441.1964

Manganese Requirement for Sporulation and Other Secondary Biosynthetic Processes of Bacillus1

Eugene D Weinberg 1
PMCID: PMC1058151  PMID: 14215975

Abstract

Concentrations of manganese, considerably in excess of the quantity required for normal vegetative growth, are needed by Bacillus for (i) synthesis of such secondary metabolites and structures as antibiotics, D-glutamyl peptide, endospores, bacteriophage, and protective antigen; and (ii) longevity of vegetative cell cultures. No other biologically active element can substitute for manganese, and no secondary biosynthetic process of Bacillus has been found in which the requirement for manganese is absent. In the present study, manganese could induce sporulation of B. megaterium even when added to synthetic broth cultures as late as 100 hr after inoculation. When sub-bactericidal concentrations of various biologically active trace elements were supplied within 2 hr of manganese addition, suppression of spore formation occurred in cultures exposed to elements of group VI (chromium, molybdenum, tungsten, selenium, tellurium) and one of group VIII (nickel); of the six interfering elements, selenium and nickel were most potent.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BERNLOHR R. W., SIEVERT C. Preliminary chemical characterization of the spore coats of Bacillus licheniformis. Biochem Biophys Res Commun. 1962 Sep 25;9:32–37. doi: 10.1016/0006-291x(62)90082-7. [DOI] [PubMed] [Google Scholar]
  2. BU'LOCK J. D. Intermediary metabolism and antibiotic synthesis. Adv Appl Microbiol. 1961;3:293–342. doi: 10.1016/s0065-2164(08)70514-8. [DOI] [PubMed] [Google Scholar]
  3. CHARNEY J., FISHER W. P., HEGARTY C. P. Managanese as an essential element for sporulation in the genus Bacillus. J Bacteriol. 1951 Aug;62(2):145–148. doi: 10.1128/jb.62.2.145-148.1951. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. COLEMAN G., ELLIOTT W. H. Studies on alpha-amylase formation by Bacillus subtilis. Biochem J. 1962 May;83:256–263. doi: 10.1042/bj0830256. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. COTZIAS G. C. Manganese in health and disease. Physiol Rev. 1958 Jul;38(3):503–532. doi: 10.1152/physrev.1958.38.3.503. [DOI] [PubMed] [Google Scholar]
  6. Foster J. W., Woodruff H. B. Bacillin, a New Antibiotic Substance from a Soil Isolate of Bacillus subtilis. J Bacteriol. 1946 Mar;51(3):363–369. [PMC free article] [PubMed] [Google Scholar]
  7. GRANT C. L., PRAMER D. Minor element composition of yeast extract. J Bacteriol. 1962 Oct;84:869–870. doi: 10.1128/jb.84.4.869-870.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. HENDLIN D. The nutritional requirements of a bacitracin-producing strain of Bacillus subtilis. Arch Biochem. 1949 Dec;24(2):435–446. [PubMed] [Google Scholar]
  9. HUYBERS K. Cationic reversion of induced phage development in Bacillus megatherium. Ann Inst Pasteur (Paris) 1953 Jan;84(1):242–253. [PubMed] [Google Scholar]
  10. KOLODZIEJ B. J., SLEPECKY R. A. A copper requirement for the sporulation of Bacillus megaterium. Nature. 1962 May 5;194:504–505. doi: 10.1038/194504a0. [DOI] [PubMed] [Google Scholar]
  11. LANKFORD C. E., KUSTOFF T. Y., SERGEANT T. P. Chelating agents in growth initiation of Bacillus globigii. J Bacteriol. 1957 Dec;74(6):737–748. doi: 10.1128/jb.74.6.737-748.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. LEONARD C. G., HOUSEWRIGHT R. D. POLYGLUTAMIC ACID SYNTHESIS BY CELL-FREE EXTRACTS OF BACILLUS LICHENIFORMIS. Biochim Biophys Acta. 1963 Jul 9;73:530–532. doi: 10.1016/0006-3002(63)90461-x. [DOI] [PubMed] [Google Scholar]
  13. LEONARD C. G., HOUSEWRIGHT R. D., THORNE C. B. Effects of metal ions on the optical specificity of glutamine synthetase and glutamyl transferase of Bacillus licheniformis. Biochim Biophys Acta. 1962 Aug 13;62:432–434. doi: 10.1016/0006-3002(62)90278-0. [DOI] [PubMed] [Google Scholar]
  14. LEONARD C. G., HOUSEWRIGHT R. D., THORNE C. B. Effects of some metallic ions on glutamyl polypeptide synthesis by Bacillus subtilis. J Bacteriol. 1958 Nov;76(5):499–503. doi: 10.1128/jb.76.5.499-503.1958. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. MAJUMDAR S. K., BOSE S. K. Trace element requirements of Bacillus subtilis for mycobacillin formation. J Bacteriol. 1960 Apr;79:564–565. doi: 10.1128/jb.79.4.564-565.1960. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. NOGUCHI Y., JOHNSON M. J. Citric acid fermentation of sugars purified with chelating resin. J Bacteriol. 1961 Oct;82:538–541. doi: 10.1128/jb.82.4.538-541.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. SLEPECKY R., FOSTER J. W. Alterations in metal content of spores of Bacillus megaterium and the effect on some spore properties. J Bacteriol. 1959 Jul;78(1):117–123. doi: 10.1128/jb.78.1.117-123.1959. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Stockton J. R., Wyss O. Proteinase Production by Bacillus subtilis. J Bacteriol. 1946 Aug;52(2):227–228. [PMC free article] [PubMed] [Google Scholar]
  19. WEINBERG E. D., BROOKS J. I. TRACE METAL CONTROL OF BACTERIAL FLAGELLATION. Nature. 1963 Aug 17;199:717–718. doi: 10.1038/199717a0. [DOI] [PubMed] [Google Scholar]
  20. WEINBERG E. D. The effect of Mn++ and antimicrobial drugs on sporulation of Bacillus subtilis in nutrient broth. J Bacteriol. 1955 Sep;70(3):289–296. doi: 10.1128/jb.70.3.289-296.1955. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. WEINBERG E. D. Trace-metal control of specific biosynthetic processes. Perspect Biol Med. 1962;5:432–445. doi: 10.1353/pbm.1962.0017. [DOI] [PubMed] [Google Scholar]
  22. WRIGHT G. G., HEDBERG M. A., SLEIN J. B. Studies on immunity in anthrax. III. Elaboration of protective antigen in a chemically defined, non-protein medium. J Immunol. 1954 Apr;72(4):263–269. [PubMed] [Google Scholar]

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