Abstract
Growth of Escherichia coli and chemical changes in the medium were very similar in highly baffled flasks and in a 50-liter fermentor run under the same oxygen-supply conditions, based on sulfite-oxidation rates. Flasks with stainless-steel baffles (Biotech) gave growth patterns and rates of glucose and NH4-N utilization almost identical to those of the fermentor; results with Bellco 598 flasks (with 6 to 7 mm deep indentations) were quite similar. Unbaffled and Bellco 600 flasks (3 to 4 mm indentations) were similar to the fermentor at very high and very low oxygen-transfer rates, but gave much less growth than the fermentor at intermediate levels. Maximal oxygen-uptake rates occurred in the fermentor at the end of the logarithmic-growth phase when growth was 40 to 75% of maximum. In the fermentor, both sulfite-oxidation rates and rates of oxygen uptake correlated reasonably well with the total amount of growth produced.
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Selected References
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- ECKER R. E., LOCKHART W. R. Specific effect of limiting nutrient on physiological events during culture growth. J Bacteriol. 1961 Oct;82:511–516. doi: 10.1128/jb.82.4.511-516.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LOCKHART W. R., SQUIRES R. W. AERATION IN THE LABORATORY. Adv Appl Microbiol. 1963;5:157–187. doi: 10.1016/s0065-2164(08)70010-8. [DOI] [PubMed] [Google Scholar]
- MCDANIEL L. E., BAILEY E. G., ZIMMERLI A. EFFECT OF OXYGEN SUPPLY RATES ON GROWTH OF ESCHERICHIA COLI. Appl Microbiol. 1965 Jan;13:109–114. doi: 10.1128/am.13.1.109-114.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Olson B. H., Johnson M. J. FACTORS PRODUCING HIGH YEAST YIELDS IN SYNTHETIC MEDIA. J Bacteriol. 1949 Feb;57(2):235–246. doi: 10.1128/jb.57.2.235-246.1949. [DOI] [PMC free article] [PubMed] [Google Scholar]
- STROHM J., DALE R. F., PEPPLER H. J. Polarographic measurement of dissolved oxygen in yeast fermentations. Appl Microbiol. 1959 Jul;7(4):235–238. doi: 10.1128/am.7.4.235-238.1959. [DOI] [PMC free article] [PubMed] [Google Scholar]