Abstract
Aflatoxins can be produced on a synthetic medium in submerged culture. Glucose, sucrose, or fructose are the preferred carbon sources, and Casamino Acids are the preferred nitrogen source. Ammonia is almost as good a nitrogen source. Zinc is required at levels of at least 0.4 mg per liter. Concentrations of aflatoxin of 60 to 80 mg per liter (as determined by optical-density measurements of a chloroform extract of the unfiltered broth) can readily be obtained in indented shake flasks; somewhat lower yields were obtained in 5-liter fermentors.
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Selected References
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- ADYE J., MATELES R. I. INCORPORATION OF LABELLED COMPOUNDS INTO AFLATOXINS. Biochim Biophys Acta. 1964 May 11;86:418–420. doi: 10.1016/0304-4165(64)90077-7. [DOI] [PubMed] [Google Scholar]
- CHANG S. B., ABDEL-KADER M. M., WICK E. L., WOGAN G. N. AFLATOXIN B2: CHEMICAL IDENTITY AND BIOLOGICAL ACTIVITY. Science. 1963 Nov 29;142(3596):1191–1192. doi: 10.1126/science.142.3596.1191. [DOI] [PubMed] [Google Scholar]
- NESBITT B. F., O'KELLY J., SARGEANT K., SHERIDAN A. Aspergillus flavus and turkey X disease. Toxic metabolites of Aspergillus flavus. Nature. 1962 Sep 15;195:1062–1063. doi: 10.1038/1951062a0. [DOI] [PubMed] [Google Scholar]
- de IONGH, BEERTHUIS R. K., VLES R. O., BARRETT C. B., ORD W. O. Investigation of the factor in groundnut meal responsible for "turkey X disease". Biochim Biophys Acta. 1962 Dec 17;65:548–551. [PubMed] [Google Scholar]