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. 1965 Mar;13(2):244–247. doi: 10.1128/am.13.2.244-247.1965

Resistance of Clostridium perfringens Type A Spores to γ-Radiation

T F Midura 1, L L Kempe 1, J T Graikoski 1, N A Milone 1
PMCID: PMC1058229  PMID: 14325887

Abstract

The radiation resistance of the spores of a classical strain and of an atypical, heat-resistant strain of Clostridium perfringens was determined. Spores were produced in Ellner's and in a Trypticase broth medium. Approximately 106 viable spores per milliliter were suspended in 0.06 m phosphate buffer and irradiated with γ rays from cobalt-60; the survivors were counted in Tryptone-yeast extract-agar by the Prickett-tube technique. Radiation D values for spores of the atypical strain in phosphate buffer and in cooked-meat broth were 0.23 and 0.30 Mrad, respectively, and the D value of the classical strain was 0.25 Mrad in phosphate buffer. Spores of the classical and atypical strains of C. perfringens type A are characterized by differences in heat resistance; yet, all strains tested demonstrated similar radiation resistance. Also, the spores were more resistant to ionizing radiation in cooked-meat broth than in phosphate buffer.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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