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. 1965 May;13(3):387–392. doi: 10.1128/am.13.3.387-392.1965

Ultracentrifugation in the Concentration and Detection of Enteroviruses

Dean O Cliver 1, John Yeatman 1
PMCID: PMC1058261  PMID: 14325278

Abstract

Ultracentrifugation has been evaluated as a method of concentrating enteroviruses from suspensions whose initial titers ranged from 1.7 × 108 to 1.6 × 10-2 plaque-forming units (PFU) per ml. A technique employing a “trap” of 0.1 ml of 2% gelatin solution at the point at which the pellet forms in tubes for the number 30 and number 50 rotors of the Spinco model L preparative ultracentrifuge has been tested and found to have a number of advantages. Qualitative studies have been performed to determine the sensitivity of the ultracentrifuge technique in detecting the presence of enteroviruses in very dilute suspensions. There was found to be at least a 50% probability of detecting virus present initially at levels as low as 0.12 PFU per ml by means of the number 50 rotor. The input level for similar results with the number 30 rotor was found to be 0.025 PFU per ml.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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