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. 1965 Jul;13(4):559–563. doi: 10.1128/am.13.4.559-563.1965

Rapid Technique for the Enumeration of Clostridium perfringens

Robert S Marshall 1,1, J Frank Steenbergen 1, L S McClung 1
PMCID: PMC1058297  PMID: 14339262

Abstract

A new medium, Tryptone-sulfite-neomycin (TSN) agar, and an incubation procedure for the enumeration of Clostridium perfringens are described. Tolerance to neomycin, optimal growth at 46 C, and sulfite-reducing properties of C. perfringens were used as a basis for development of the medium. Comparisons were made between sulfite-polymyxin-sulfadiazine (SPS) agar and TSN agar at 37 and 46 C with C. perfringens and other organisms. These studies indicate the quantitative and selective superiority of TSN agar, incubated at 46 C, over SPS agar.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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