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. 1966 Nov;14(6):966–972. doi: 10.1128/am.14.6.966-972.1966

Production of Enterotoxin A

Eiichi Kato 1,1, Mahmood Khan 1, Lubinka Kujovich 1, Merlin S Bergdoll 1
PMCID: PMC1058450  PMID: 16349703

Abstract

A method for production of enterotoxin A in multiple liter lots is described. The medium contained 4% N-Z Amine NAK supplemented with 0.001% niacin and 0.00005% thiamine, and was adjusted to pH 6. The inoculated medium in lots of 400 to 600 ml, in 2-liter Erlenmeyer flasks, was incubated at 37 C for 24 hr on a gyrotory shaker at 280 rev/min. Production of 4 to 6 μg of enterotoxin A per ml occurred.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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