Abstract
An anaerobic system is described which allows the microbiologist or hospital technician ease of operation not previously possible with other systems. Gas cylinders, vacuum pumps, valves, and gauges have been eliminated. A new anaerobic lid was developed that is fitted only with a snap-in rubber gasket, a double stainless-steel gauze flash arrestor, and a catalyst holder. The holder contains a palladium pellet catalyst which is active at room temperature and requires no heat for activation. This system was made specifically for use with a disposable hydrogen-carbon dioxide generator and a disposable methylene blue anaerobic indicator. In addition to ease of operation, this jar incorporates unique safety features which eliminate the possibility of a laboratory explosion. An oxygen-free atmosphere composed primarily of nitrogen and carbon dioxide was quickly achieved within the jar to insure maximal growth.
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Selected References
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- BREWER J. H., HEER A. A., McLAUGHLIN C. B. The use of sodium borohydride for producing hydrogen in an anaerobe jar. Appl Microbiol. 1955 May;3(3):136–136. doi: 10.1128/am.3.3.136-136.1955. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Brewer J. H., Allgeier D. L. Disposable Hydrogen Generator. Science. 1965 Feb 26;147(3661):1033–1034. doi: 10.1126/science.147.3661.1033-a. [DOI] [PubMed] [Google Scholar]
- Brewer J. H., Allgeier D. L., McLaughlin C. B. Improved anaerobic indicator. Appl Microbiol. 1966 Jan;14(1):135–136. doi: 10.1128/am.14.1.135-136.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KHAIRAT O. MODERNIZING BREWER AND OTHER ANAEROBIC JARS. J Bacteriol. 1964 Apr;87:963–964. doi: 10.1128/jb.87.4.963-964.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]