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. 1981 Aug 25;9(16):4189–4199. doi: 10.1093/nar/9.16.4189

Reiterated sequences within the intron of an immediate-early gene of herpes simplex virus type 1.

R J Watson, K Umene, L W Enquist
PMCID: PMC1058475  PMID: 6272198

Abstract

We describe the nucleotide sequence of a herpes simplex virus type 1 DNA fragment containing the intron of the immediate-early mRNA-5 (IE mRNA-5) gene. The location of the intron within this fragment was determined by a Berk & Sharp nuclease S1 protection analysis, and by cloning and sequencing cDNA containing sequences overlapping t he IE mRNA-5 splice point. We found that the 149 base pair (bp) intron contained four copies of an identical 23 bp GC rich tandem repeat followed by a further reiteration consisting of the first 15 bp only.

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