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. 2023 Oct 20;12(1):2269634. doi: 10.1080/2162402X.2023.2269634

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© 2023 The Author(s). Published with license by Taylor & Francis Group, LLC.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The terms on which this article has been published allow the posting of the Accepted Manuscript in a repository by the author(s) or with their consent.

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Figure 4. — Lin- cells are not the main source of IL-22 in established murine liver metastasis.

(a) Gating strategy used to assess the source of CD45+IL-22+ cells isolated from mouse healthy and metastatic liver upon i.s. injection of MC38 cells. (b) Diagram showing the proportion of CD45+IL-22+ cells in healthy and metastatic murine liver. (c) Gating strategy used to identify lymphocytes isolated from mouse healthy and metastatic liver upon i.s. injection of MC38 cells. (d) Flow cytometry of isolated Lin+ and Lin- lymphocytes of healthy and metastatic mouse liver upon i.s. injection of MC38 cells and respective quantification. n ≥ 4 mice per group. Data presented as mean ± SEM. ns > 0.05; **:p ≤ 0.01 as assessed by Mann-Whitney U test.