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. Author manuscript; available in PMC: 2024 Oct 18.
Published in final edited form as: Neuron. 2023 Aug 4;111(20):3176–3194.e7. doi: 10.1016/j.neuron.2023.07.005

Figure 5: Chronic suppression of γ-secretase activity reduces plasma membrane cholesterol in human neurons.

Figure 5:

(A) Representative images illustrating reduced Filipin staining (blue) in human neurons upon chronic suppression of γ-secretase activity with DAPT or LY411,575. Neurons are labeled by GFP expression (green), and axons are labeled by L1CAM staining (red). Scale bars (white line) represent 10 μm.

(B) Chronic suppression of γ-secretase activity with DAPT or LY411,575 causes a robust decrease in the plasma membrane cholesterol content of human neurons as monitored by Filipin signal in GFP-positive human neurons.

(C & D) Same as A & B, but for PSEN1 KO neurons obtained from three independent KO lines.

Data in (B) and (D) are means ± SEM (numbers of cells/biological replicates) are shown in bars. Statistical analyses were conducted using two-tailed unpaired t-test, with * = p<0.05; ** = p<0.01, *** = p<0.001.