Figure 2. STIM2 loss enhances tumor growth and metastasis in mice.
(A) Schematic representation of intrasplenic injection of luciferase tagged HCT116, STIM1 KO #01, STIM2 KO #15, and DKO #22 clones of HCT116 cells. The cells were injected at 5 ×105 cells/mice into the spleen of male NOD-SCID mice.
(B, C) Male NOD-SCID mice injected with luciferase tagged HCT116, STIM1 KO #01, STIM2 KO #15, and DKO #22 clones of HCT116 cells (B) representative image with three mice per group, and (C) quantification of whole-body luminance (Scale bar 2.0 in).
(D, E) Six weeks after intrasplenic injection of HCT116, STIM1 KO #01, STIM2 KO #15, and DKO #22 clones of HCT116 cells, the mice were sacrificed, and the primary tumor at the injection site was imaged, (D) representative image of primary tumors, and (E) quantification of primary tumor weight. (Scale bar 0.5 cm).
(F-I) Post intrasplenic injection of HCT116, STIM1 KO #01, STIM2 KO #15, and DKO #22 clones of HCT116 cells, organs of intrasplenic injected mice were harvested, and luminance was measured (F) representative image of liver, lung, and colon showing metastasis, and quantification of total luminance in (G) liver (Scale bar 1 cm), (H) lung (Scale bar 0.5 cm), and (I) colon (Scale bar 0.5 cm).
(J) Survival of NOD-SCID mice injected with HCT116, STIM1 KO #01, STIM2 KO #15, and STIM DKO #22 clones of HCT116 cells (p< 0.0003). ANOVA followed with a post hoc Tukey test to compare between groups. *p<0.05, **p<0.01 and ***p<0.001