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. 2023 Oct 10;9(10):e20556. doi: 10.1016/j.heliyon.2023.e20556

Fig. 4.

Fig. 4

Reduction of TLR3 inflammatory signaling pathway protein by inotodiol in poly(I: C)-induced inflammation model. HDF cell was pre-treated Inotodiol dose-dependently with serum-free media 5 h and incubated poly(I:C) 10 μg/ml for 24 h and protein Lysis for Western blot analysis (A) Represent data with indicated phospho-antibodies, and TLR3, NFκB, IκB and actin antibodies. (B) Relative protein expression was quantified using Image J. data are presented in mean values ± SD. #p < 0.05; ##p < 0.005; ####p < 0.0001 vs. the control group. *p < 0.05; **p < 0.005; ****p < 0.0001 vs. the poly(I:C) group.