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. 2023 Oct 4;622(7984):834–841. doi: 10.1038/s41586-023-06611-6

Fig. 1. Eosin-positive structures consisting of haemoglobin in the cartilaginous tissues.

Fig. 1

a, Haematoxylin and eosin (H&E) staining indicate eosin-positive structures (black arrows) in the hypertrophic chondrocytes of the cartilaginous growth plate of P7 mice (zoomed-in images of the middle and right top panels), which were confirmed by scanning electron microscopy (SEM) (white arrows; bottom panels). The blue arrows indicate RBCs in the bone marrow. Scale bars, 10 μm. b, The eosin-positive structures (black arrows) in hypertrophic chondrocytes of ribs (top panels) and calcaneus (bottom panels) of E15.5 mice. Scale bars, 10 μm. c, The presence of eosinophilic structures (black arrows) in chondrocytes of the resting, proliferative and hypertrophic zones of growth plates from E18.5 mice by Masson trichrome staining. The blue arrows indicate nuclei. Scale bars, 10 μm. d, The presence of eosinophilic structures (black arrows) in the non-hypertrophic zone of femoral articular cartilage from a man 16 years of age (zoomed-in images of the middle and right top panels), which were confirmed by SEM (white arrows; bottom panels). The blue arrows indicate RBCs in the bone marrow. Scale bars, 10 μm. e, Immunohistochemistry (top panels) and immunoelectron microscope staining (iTEM; bottom panels) of HBA and HBB in mouse humeral cartilage from P3 mice. Scale bars, 50 μm (top and middle row panels) and 5 μm (iTEM). f, The expression of HBA and HBB in chondrocytes isolated from P7 mouse humeral cartilage as detected by immunohistochemistry staining. The black arrows indicate haemoglobin. Scale bar, 50 μm.