Extended Data Fig. 7. Regulation of hemoglobin expression by Klf1.
a, Klf1-LoxP qPCR of genomic DNA extracted from Klf1F/F(Ctrl) or Klf1F/F/Col2a1-CreERT2 (Klf1-cKO) growth plates of P5 mice, which were treated by tamoxifen (100 mg/kg) for 4 days. Data were normalized to β2-microglobulin (n = 3 biologically independent samples). b, Quantification of Klf1 protein by Western blot analysis of total protein lysate extracted from Ctrl or Klf1-cKO primary cultured chondrocytes. A representative Western blot is shown on the left, and quantification of all biological replicates is provided on the right. Data were normalized to α-tubulin (n = 3 biologically independent samples). For gel source data, see Supplementary Fig. 1i.c, d Expression of Klf1, Hba, Hbb, Hif-1α and Hif-2α in the primary chondrocytes upon Klf1 depletion by RNA interference in either 20% (c) or 1% (d) O2. n = 3 biologically independent experinmets. e, Quantification of Klf1 protein by Western blot analysis of total protein lysate extracted from Ctrl or Klf1 depletion by RNA interference primary cultured chondrocytes in either 20% or 1% O2. A representative Western blot is shown on the left, and quantification of all biological replicates is provided on the right. Data were normalized to α-tubulin (n = 3 biologically independent experinmets). For gel source data, see Supplementary Fig. 1j. f, Expression of Hba, Hbb, Hif-1α and Hif-2α in ATDC5 chondrocyte cell lines upon Klf1 knockdown as examined by quantitative PCR. The data are mean with SEM of triplicate experiments. Error bars represent SEM. P values were calculated using one-way ANOVA tests (a-f). The exact P-values of comparison are presented in the figures, respectively.