Fig. 3 |. Propionate and vitamin B₁₂ supplementation restore the growth of PDIM(+) Mtb.

a, Relative growth of the PDIM reference strain set in 7H9/OADC/glycerol/tyloxapol + PALM media with increasing concentrations of propionate compared to no propionate controls. Mean ± SD for n = 3 biological replicates. b–e, Growth curves of PDIM(+) and PDIM(−) Mtb H37Rv in (b) standard 7H9/OADC/glycerol/tyloxapol and (c) supplemented with 0.1 mM propionate, (d) 7.4 μM vitamin B₁₂ (10 μg/ml), or (e) 0.1 mM cholesterol. Mean ± SD for n = 3 biological replicates. **P < 0.01, ***P < 0.001, ****P < 0.0001 for both wt and comp versus ΔppsD; two-way ANOVA with šidák’s multiple comparison test. Data in (a–e) are representative of at least two independent experiments. For some data points the SD is smaller than the data symbols. f, Abundance of methylmalonyl-CoA (MMCoA) in PDIM(+) and PDIM(−) H37Rv grown in standard 7H9/OADC/glycerol/tyloxapol media ± 0.1 mM propionate, and g, PDIM(+) H37Rv wildtype in standard media and supplemented with either propionate or vitamin B₁₂. Abundances are shown as normalized area under the curve (AUC). Mean ± SD for n = 6 biological replicates from two independent experiments. *P < 0.05, **P < 0.01, ****P < 0.0001; one-way ANOVA with Tukey’s multiple comparison test. Significant differences between ± propionate for each strain and between strains for each condition are indicated in (f), and compared to unsupplemented media in (g).