Extended Data Fig. 1 |. Resistance of PDIM(−) and PDIM(+) Mtb to high molecular weight compounds.

a–g, MIC assays of Mtb mc²7902 [PDIM(+)] and mc²8398 [PDIM(−)] to (a) ramoplanin (RAM), (b) teicoplanin (TEC), (c) vancomycin (VAN), (d) rifampicin (RIF), (e) azithromycin (AZM), (f) erythromycin (ERY), and (g) isoniazid (INH). Compounds are arranged by descending molecular weight, which is shown on the MIC plots. MICs were performed in 7H9/OADC/glycerol/tyloxapol + PALM media and bacterial growth was measured after 10 days of incubation and normalized to drug-free controls. Mean ± SD for n = 4 biological replicates from two independent experiments. h, Ethidium Bromide uptake of mc²7902 and mc²8398. Uptake in whole cell suspensions was monitored by fluorescence (Ex 355 nm/Em 590 nm). Mean ± SD for n = 4 biological replicates, each measured in five technical replicates. Uptake data are representative of two independent experiments. *P < 0.001; two-way ANOVA with šidák’s multiple comparison test.