KEY RESOURCES TABLE

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Mouse monoclonal anti-His	Transgen Biotech	HT501–01
Goat anti-mouse antibody	CWBIO	CW0102
Bacterial and virus strains
Escherichia coli (E. coli) DH5α competent cell	Lab stock	N/A
E. coli BL21 (DE3) competent cell	Transgen Biotech	CD601–02
Rhizobium radiobacter (Agrobacterium tumefaciens) GV3101 pMP90 competent cell	Lab stock	N/A
Chemicals, peptides, and recombinant proteins
Biotin-pRALF11/26, Biotin-sRALF22/33, RALF4, RALF7	Scilight Biotechnology LLC (Beijing, China)	N/A
Biotin-elf24	Scilight Biotechnology LLC (Beijing, China)	N/A
pRALF11/26, sRALF33	Scilight Biotechnology LLC (Beijing, China)	N/A
KI	Sangon Biotech	A100512–0050
CuCl2	Sangon Biotech	A603090–0250
DPI	Meilunbio	MB1817–1
H2DCF-DA	Invitrogen	C6827
FER-6×His	This paper	N/A
CVY1–6×His	This paper	N/A
ANJ-6×His	This paper	N/A
HERK1–6×His	This paper	N/A
LRR4–6×His	This paper	N/A
Critical commercial assays
Gateway Cloning Kit	Invitrogen
Deposited data
N/A	N/A	N/A
Experimental models: Cell lines
N/A	N/A	N/A
Experimental models: Organisms/strains
Arabidopsis: Col-0	Lab stock	N/A
Arabidopsis: r8 r9 r15	This paper	N/A
Arabidopsis: r10 r11 r12 r13	This paper	N/A
Arabidopsis: r25 r26 r30	This paper	N/A
Arabidopsis: ralf sept	This paper	N/A
Arabidopsis: r33–3	Liu et al.16	N/A
Arabidopsis: r22 r33	This paper	N/A
Arabidopsis :r1 r22 r33	This paper	N/A
Arabidopsis: ralf quad	This paper	N/A
Arabidopsis: fer-4	Duan et al.35	N/A
Arabidopsis: anj herk1	Galindo-Trigo et al.37	N/A
Arabidopsis: cvy1	Lab stock	N/A
Arabidopsis: the1-4	Lab stock	N/A
Arabidopsis: cvy1 anj herk1	This paper	N/A
Arabidopsis: lrx3/4/5	Zhao et al.50	N/A
Arabidopsis lyrata	From Ya-Long Guo	N/A
Capsella rubella	From Ya-Long Guo	N/A
Cardamine flexuosa	Lab stock	N/A
Rorippa indica	Lab stock	N/A
Erysium cheiranthoides	Lab stock	N/A
Descurainia sophia	Lab stock	N/A
Oligonucleotides
Primers used in this study are listed in Table S1	This paper	N/A
Recombinant DNA
Plasmid: pR11/pR26pro:GUS	This paper	N/A
Plasmid: pR11/pR26pro: pR11/pR26-GFP	This paper	N/A
Plasmid: pR11/pR26pro: pR11/pR26-LAT52:GFP	This paper	N/A
Plasmid: FER/ANJ/HERK1/CVY 1pro:GUS	This paper	N/A
Plasmid: pHEE401E-RALF1-22-23-33	This paper	N/A
Plasmid: pHEE401E-RALF10–13	This paper	N/A
Plasmid: pHEE401E-RALF25-26-30	This paper	N/A
Plasmid: pHEE401E-CVY1	This paper	N/A
Plasmid: pET28GW-LRR4	This paper	N/A
Software and algorithms
ImageJ	N/A	https://imagei.nih.gov/ii/
cellSens	N/A	https://lifescience.evidentscientific.com.cn/en/software/cellsens/
Bioedit	N/A	https://bioedit.software.informer.com/7.2/
R version 4.1.3	N/A	https://www.r-proiect.org/
MEGA X	N/A	https://megasoftware.net/