Skip to main content
NCBI home page
Plant Physiology logoLink to Plant Physiology
. 1989 May;90(1):37–40. doi: 10.1104/pp.90.1.37

DNA Methylation and Embryogenic Competence in Leaves and Callus of Napiergrass (Pennisetum purpureum Schum.) 1

Fionnuala M Morrish 1, Indra K Vasil 1
PMCID: PMC1061672  PMID: 16666764

Abstract

Quantitative and qualitative levels of DNA methylation were evaluated in leaves and callus of Pennisetum purpureum Schum. The level of methylation did not change during leaf differentiation or aging and similar levels of methylation were found in embryogenic and nonembryogenic callus.

Full text

PDF
37

Images in this article

38Figure 1
on p.38
38Figure 2
on p.38

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cannon F. C., Dixon R. A., Postgate J. R., Primrose S. B. Chromosomal integration of Klebsiella nitrogen fixation genes in Escherichia coli. J Gen Microbiol. 1974 Jan;80(1):227–239. doi: 10.1099/00221287-80-1-227. [DOI] [PubMed] [Google Scholar]
  2. Eichholtz D. A., Rogers S. G., Horsch R. B., Klee H. J., Hayford M., Hoffmann N. L., Braford S. B., Fink C., Flick J., O'Connell K. M. Expression of mouse dihydrofolate reductase gene confers methotrexate resistance in transgenic petunia plants. Somat Cell Mol Genet. 1987 Jan;13(1):67–76. doi: 10.1007/BF02422300. [DOI] [PubMed] [Google Scholar]
  3. Gehrke C. W., Kuo K. C., McCune R. A., Gerhardt K. O., Agris P. F. Quantitative enzymatic hydrolysis of tRNAs: reversed-phase high-performance liquid chromatography of tRNA nucleosides. J Chromatogr. 1982 Jul 9;230(2):297–308. [PubMed] [Google Scholar]
  4. Gehrke C. W., McCune R. A., Gama-Sosa M. A., Ehrlich M., Kuo K. C. Quantitative reversed-phase high-performance liquid chromatography of major and modified nucleosides in DNA. J Chromatogr. 1984 Sep 28;301(1):199–219. doi: 10.1016/s0021-9673(01)89189-5. [DOI] [PubMed] [Google Scholar]
  5. Gruenbaum Y., Naveh-Many T., Cedar H., Razin A. Sequence specificity of methylation in higher plant DNA. Nature. 1981 Aug 27;292(5826):860–862. doi: 10.1038/292860a0. [DOI] [PubMed] [Google Scholar]
  6. Hanam S. R., Dale S. J. Subluxation of the peroneal tendons. A report of two cases. J Am Podiatr Med Assoc. 1986 May;76(5):286–291. doi: 10.7547/87507315-76-5-286. [DOI] [PubMed] [Google Scholar]
  7. Holliday R. The inheritance of epigenetic defects. Science. 1987 Oct 9;238(4824):163–170. doi: 10.1126/science.3310230. [DOI] [PubMed] [Google Scholar]
  8. McClelland M., Nelson M. The effect of site specific methylation on restriction endonuclease digestion. Nucleic Acids Res. 1985;13 (Suppl):r201–r207. doi: 10.1093/nar/13.suppl.r201. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Rajasekaran K., Hein M. B., Vasil I. K. Endogenous Abscisic Acid and Indole-3-Acetic Acid and Somatic Embryogenesis in Cultured Leaf Explants of Pennisetum purpureum Schum. : Effects in Vivo and in Vitro of Glyphosate, Fluridone, and Paclobutrazol. Plant Physiol. 1987 May;84(1):47–51. doi: 10.1104/pp.84.1.47. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Roberts R. J. Restriction and modification enzymes and their recognition sequences. Nucleic Acids Res. 1980 Jan 11;8(1):r63–r80. doi: 10.1093/nar/8.1.197-d. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Spena A., Viotti A., Pirrotta V. Two adjacent genomic zein sequences: structure, organization and tissue-specific restriction pattern. J Mol Biol. 1983 Oct 5;169(4):799–811. doi: 10.1016/s0022-2836(83)80137-5. [DOI] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES