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. 1989 Jun;90(2):398–400. doi: 10.1104/pp.90.2.398

Quantitation of Indoleacetic Acid Conjugates in Bean Seeds by Direct Tissue Hydrolysis 1

Krystyna Bialek 1,2, Jerry D Cohen 1,2
PMCID: PMC1061736  PMID: 16666783

Abstract

Gas chromatography-selected ion monitoring-mass spectral analysis using [13C6]indole-3-acetic acid (IAA) as an internal standard provides an effective means for quantitation of IAA liberated during direct strong basic hydrolysis of bean (Phaseolus vulgaris L.) seed powder, provided that extra precautions are undertaken to exclude oxygen from the reaction vial. Direct seed powder hydrolysis revealed that the major portion of amide IAA conjugates in bean seeds are not extractable by aqueous acetone, the solvent used commonly for IAA conjugate extraction from seeds and other plant tissues. Strong basic hydrolysis of plant tissue can be used to provide new information on IAA content.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bandurski R. S., Schulze A. Concentration of Indole-3-acetic Acid and Its Derivatives in Plants. Plant Physiol. 1977 Aug;60(2):211–213. doi: 10.1104/pp.60.2.211. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bialek K., Cohen J. D. Isolation and Partial Characterization of the Major Amide-Linked Conjugate of Indole-3-Acetic Acid from Phaseolus vulgaris L. Plant Physiol. 1986 Jan;80(1):99–104. doi: 10.1104/pp.80.1.99. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Chen K. H., Miller A. N., Patterson G. W., Cohen J. D. A Rapid and Simple Procedure for Purification of Indole-3-Acetic Acid Prior to GC-SIM-MS Analysis. Plant Physiol. 1988 Mar;86(3):822–825. doi: 10.1104/pp.86.3.822. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Chisnell J. R. Myo-inositol esters of indole-3-acetic acid are endogenous components of Zea mays L. shoot tissue. Plant Physiol. 1984;74:278–283. doi: 10.1104/pp.74.2.278. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cohen J. D., Baldi B. G., Slovin J. P. C(6)-[benzene ring]-indole-3-acetic Acid: a new internal standard for quantitative mass spectral analysis of indole-3-acetic Acid in plants. Plant Physiol. 1986 Jan;80(1):14–19. doi: 10.1104/pp.80.1.14. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Cohen J. D. Identification and Quantitative Analysis of Indole-3-Acetyl-l-Aspartate from Seeds of Glycine max L. Plant Physiol. 1982 Sep;70(3):749–753. doi: 10.1104/pp.70.3.749. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Epstein E., Baldi B. G., Cohen J. D. Identification of Indole-3-Acetylglutamate from Seeds of Glycine max L. Plant Physiol. 1986 Jan;80(1):256–258. doi: 10.1104/pp.80.1.256. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Felker P. A gas--liquid chromatographic-isotope dilution analysis of cysteine, histidine, and tryptophan in acid-hydrolyzed protein. Anal Biochem. 1976 Nov;76(50):192–213. doi: 10.1016/0003-2697(76)90278-5. [DOI] [PubMed] [Google Scholar]
  9. Percival F. W., Bandurski R. S. Esters of indole-3-acetic Acid from Avena seeds. Plant Physiol. 1976 Jul;58(1):60–67. doi: 10.1104/pp.58.1.60. [DOI] [PMC free article] [PubMed] [Google Scholar]

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