Skip to main content
NCBI home page
Plant Physiology logoLink to Plant Physiology
. 1989 Jun;90(2):728–733. doi: 10.1104/pp.90.2.728

Reduced Accumulation of ABA during Water Stress in a Molybdenum Cofactor Mutant of Barley 1

Mary Walker-Simmons 1,2, David A Kudrna 1,2, Robert L Warner 1,2
PMCID: PMC1061788  PMID: 16666835

Abstract

A barley (Hordeum vulgare L.) mutant (Az34) has been identified with low basal levels of abscisic acid (ABA) and with reduced capacity for producing ABA in response to water stress. The mutation is in a gene controlling the molybdenum cofactor resulting in a pleiotropic deficiency in at least three molybdoenzymes, nitrate reductase, xanthine dehydrogenase, and aldehyde oxidase. The mutant was found to lack aldehyde oxidase activity with several substrates including: (a) ABA aldehyde, a putative precursor of ABA; (b) an acetylenic analog of ABA aldehyde; and (c) heptaldehyde. Elevating the growth temperature from 18 to 26°C caused mutant leaves to wilt and brown. Desiccation of mutant leaves was prevented by applying ABA. These results indicate that ABA biosynthesis at some developmental stages is dependent upon a molybdoenzyme which may be an aldehyde oxidase.

Full text

PDF
728

Images in this article

731Figure 5
on p.731

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Creelman R. A., Zeevaart J. A. Incorporation of oxygen into abscisic Acid and phaseic Acid from molecular oxygen. Plant Physiol. 1984 May;75(1):166–169. doi: 10.1104/pp.75.1.166. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Gómez J., Sánchez-Martínez D., Stiefel V., Rigau J., Puigdomènech P., Pagès M. A gene induced by the plant hormone abscisic acid in response to water stress encodes a glycine-rich protein. Nature. 1988 Jul 21;334(6179):262–264. doi: 10.1038/334262a0. [DOI] [PubMed] [Google Scholar]
  3. Johnson J. L., Rajagopalan K. V. Structural and metabolic relationship between the molybdenum cofactor and urothione. Proc Natl Acad Sci U S A. 1982 Nov;79(22):6856–6860. doi: 10.1073/pnas.79.22.6856. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Kan K. S., Thornber J. P. The Light-harvesting Chlorophyll a/b-Protein Complex of Chlamydomonas reinhardii. Plant Physiol. 1976 Jan;57(1):47–52. doi: 10.1104/pp.57.1.47. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Kramer S. P., Johnson J. L., Ribeiro A. A., Millington D. S., Rajagopalan K. V. The structure of the molybdenum cofactor. Characterization of di-(carboxamidomethyl)molybdopterin from sulfite oxidase and xanthine oxidase. J Biol Chem. 1987 Dec 5;262(34):16357–16363. [PubMed] [Google Scholar]
  6. Mundy J., Chua N. H. Abscisic acid and water-stress induce the expression of a novel rice gene. EMBO J. 1988 Aug;7(8):2279–2286. doi: 10.1002/j.1460-2075.1988.tb03070.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. PATEMAN J. A., COVE D. J., REVER B. M., ROBERTS D. B. A COMMON CO-FACTOR FOR NITRATE REDUCTASE AND XANTHINE DEHYDROGENASE WHICH ALSO REGULATES THE SYNTHESIS OF NITRATE REDUCTASE. Nature. 1964 Jan 4;201:58–60. doi: 10.1038/201058a0. [DOI] [PubMed] [Google Scholar]
  8. Raschke K., Zeevaart J. A. Abscisic Acid Content, Transpiration, and Stomatal Conductance As Related to Leaf Age in Plants of Xanthium strumarium L. Plant Physiol. 1976 Aug;58(2):169–174. doi: 10.1104/pp.58.2.169. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Sindhu R. K., Walton D. C. Conversion of xanthoxin to abscisic Acid by cell-free preparations from bean leaves. Plant Physiol. 1987 Dec;85(4):916–921. doi: 10.1104/pp.85.4.916. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Sindhu R. K., Walton D. C. Xanthoxin Metabolism in Cell-free Preparations from Wild Type and Wilty Mutants of Tomato. Plant Physiol. 1988 Sep;88(1):178–182. doi: 10.1104/pp.88.1.178. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Somers D. A., Kuo T. M., Kleinhofs A., Warner R. L. Nitrate reductase-deficient mutants in barley : immunoelectrophoretic characterization. Plant Physiol. 1983 Jan;71(1):145–149. doi: 10.1104/pp.71.1.145. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Walker-Simmons M. ABA Levels and Sensitivity in Developing Wheat Embryos of Sprouting Resistant and Susceptible Cultivars. Plant Physiol. 1987 May;84(1):61–66. doi: 10.1104/pp.84.1.61. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Zeevaart J. A. Sites of Abscisic Acid Synthesis and Metabolism in Ricinus communis L. Plant Physiol. 1977 May;59(5):788–791. doi: 10.1104/pp.59.5.788. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES