Skip to main content
NCBI home page
Plant Physiology logoLink to Plant Physiology
. 1989 Jul;90(3):867–875. doi: 10.1104/pp.90.3.867

Electrolyte Leakage, Lipoxygenase, and Lipid Peroxidation Induced in Tomato Leaf Tissue by Specific and Nonspecific Elicitors from Cladosporium fulvum1

Tobin L Peever 1,2, Verna J Higgins 1
PMCID: PMC1061813  PMID: 16666890

Abstract

Glycoprotein nonspecific elicitor (NSE) and a specific elicitor preparation from intercellular fluids (SE) of tomato (Lycopersicon esculentum Mill. cv Bonny Best or Potentate) infected with race 2.4.5 of Cladosporium fulvum Cooke [syn. Fulvia fulva (Cooke) Ciferri] were injected into cv Sonatine (resistant to race 2.4.5) to compare electrolyte leakage, lipoxygenase activity, and lipid peroxidation induced in response to these elicitors. Increased electrolyte leakage was induced by NSE or SE; the leakage due to NSE but not to SE was inhibited by the nonsteroidal antiinflammatory drug (NSAID) piroxicam. Under normal photoperiod conditions, higher levels of lipoxygenase activity were detected 6 hours after injection with either elicitor. This activity peaked by 12 hours with both elicitors and declined to control levels by 24 hours when visible necrosis could be detected. Both NSE and SE-induced lipoxygenase was inhibited by piroxicam in vitro. Lipid peroxidation in elicitor-treated tissue was also assayed at 6, 12, and 24 hours after injection using the TBA test for malonaldehyde. Increased peroxidation was detected in response to NSE or SE at 12 hours with similar values obtained at 24 hours. With plants incubated in the dark, lipoxygenase, and lipid peroxidation were similarly induced in SE-injected tissue whereas necrosis induction by SE was light dependent.

Full text

PDF
867

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ben-Aziz A., Grossman S., Ascarelli I., Budowski P. Linoleate oxidation induced by lipoxygenase and heme proteins: a direct spectrophotometric assay. Anal Biochem. 1970 Mar;34:88–100. doi: 10.1016/0003-2697(70)90089-8. [DOI] [PubMed] [Google Scholar]
  2. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1016/0003-2697(76)90527-3. [DOI] [PubMed] [Google Scholar]
  3. Daub M. E. Peroxidation of tobacco membrane lipids by the photosensitizing toxin, cercosporin. Plant Physiol. 1982 Jun;69(6):1361–1364. doi: 10.1104/pp.69.6.1361. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Galliard T., Phillips D. R. Lipoxygenase from potato tubers. Partial purification and properties of an enzyme that specifically oxygenates the 9-position of linoleic acid. Biochem J. 1971 Sep;124(2):431–438. doi: 10.1042/bj1240431. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Grossman S., Zakut R. Determination of the activity of lipoxygenase (lipoxidase). Methods Biochem Anal. 1979;25:303–329. doi: 10.1002/9780470110454.ch5. [DOI] [PubMed] [Google Scholar]
  6. Rogers K. R., Albert F., Anderson A. J. Lipid peroxidation is a consequence of elicitor activity. Plant Physiol. 1988 Feb;86(2):547–553. doi: 10.1104/pp.86.2.547. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Rogers K. R., Anderson A. J. The Effect of Extracellular Components from Colletotrichum lindemuthianum on Membrane Transport in Vesicles Isolated from Bean Hypocotyl. Plant Physiol. 1987 Jun;84(2):428–432. doi: 10.1104/pp.84.2.428. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Sircar J. C., Schwender C. F., Johnson E. A. Soybean lipoxygenase inhibition by nonsteroidal antiinflammatory drugs. Prostaglandins. 1983 Mar;25(3):393–396. doi: 10.1016/0090-6980(83)90042-4. [DOI] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES