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. 1989 Jul;90(3):921–927. doi: 10.1104/pp.90.3.921

Interruption of Somatic Embryogenesis in Daucus carota L. by 5-Bromodeoxyuridine

John C Thomas 1,2,3, Craig Nessler 1,2,3, Frank Katterman 1,2,3
PMCID: PMC1061821  PMID: 16666898

Abstract

Embryogenic Daucus carota L. cells grown in 9 micromolar 2.4-dichlorophenoxyacetic acid are resistant to greater than 5 micromolar 5-bromodeoxyuridine (BrdU). In contrast, 5 micromolar BrdU strongly inhibits somatic embryogenesis within 24 hours after transfer of cells to an auxin-free medium. DNA synthesis rates in control and BrdU-treated cultures are rapid and similar; however, the DNA content does not reach levels as great in the presence of BrdU as in control cultures. BrdU substitutes for thymidine in the DNA in 28% of the available sites 48 hours after auxin removal. Following DNA repair, somatic embryogenesis resumes. BrdU DNA incorporation leads to somatic embryogenesis inhibition and provides an alternative to auxin treatment for the interruption of carrot cell culture differentiation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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