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. 1989 Aug;90(4):1310–1315. doi: 10.1104/pp.90.4.1310

Superoxide Dismutase Activity in Needles of Norwegian Spruce Trees (Picea abies L.) 1

Andrea Polle 1,2, Brigitte Krings 1,2, Heinz Rennenberg 1,2
PMCID: PMC1061888  PMID: 16666928

Abstract

The activity of superoxide dismutase was investigated in needles of spruce trees. To obtain maximum activity, needles were homogenized in the presence of Triton X-100 and polyvinylpyrrolidone. Superoxide dismutase activity was measured in dialyzed extracts with a modified epinephrine assay (HP Misra, I Fridovich [1972] J Biol Chem 247: 3170-3175) at pH 10.2. The extracts contained 70 to 120 units of superoxide dismutase per milligram protein. One unit of superoxide dismutase was completely inhibited in the presence of 20 micromolar NaCN. On native polyacrylamide gels three electromorphs were visualized after staining for activity. All three species were sensitive to CN− and H2O2 and were therefore assumed to be Cu/Zn-superoxide dismutases. Superoxide dismutase activity was dependent on the age of the needles and declined by approximately 25% within 3 to 4 years.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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