Skip to main content
PMC home page
Plant Physiology logoLink to Plant Physiology
. 1989 Aug;90(4):1359–1364. doi: 10.1104/pp.90.4.1359

Post-Transcriptional Control of Sucrose Synthase Expression in Anaerobic Seedlings of Maize 1

Earl W Taliercio 1,2,3, Prem S Chourey 1,2,3
PMCID: PMC1061896  PMID: 16666936

Abstract

We have examined post-transcriptional control of expression of the anaerobically induced sucrose synthase 1 (SS1) isozyme mRNA encoded by the shrunken (Sh) gene of maize (Zea mays L.). The SS1 transcript level is increased in maize seedling roots during anaerobiosis without a concomitant increase in the SS1 protein level. We show that the anaerobic SS1 RNA was loaded onto polyribosomes and that SS1 proteins produced by in vitro translation of polyribosomal RNA from anaerobic roots and immature kernels were indistinguishable based on abundance and apparent molecular weight. [35S]Methionine uptake in control and anaerobically stressed seedling roots indicated a detectable, but only slight, increase in radiolabel in the SS1 polypeptide as compared to the sucrose synthase 2 isozyme, SS2. However, this slight increase in [35S]methionine uptake did not contribute to a detectable increase in the steady state level of SS1 protein relative to SS2 protein. Chase experiments with unlabeled methionine indicated that SS1 protein was relatively stable in the anaerobic environment. From these results we conclude that SS1 protein was not rapidly turned over in the anaerobic environment and that expression of anaerobically induced SS1 transcripts was blocked at some step beyond polyribosomal loading.

Full text

PDF
1359

Images in this article

1360Figure 1
on p.1360
1361Figure 2
on p.1361
1361Figure 3
on p.1361
1362Figure 4
on p.1362

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bracey L. T., Paigen K. Changes in translational yield regulate tissue-specific expression of beta-glucuronidase. Proc Natl Acad Sci U S A. 1987 Dec;84(24):9020–9024. doi: 10.1073/pnas.84.24.9020. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Chirgwin J. M., Przybyla A. E., MacDonald R. J., Rutter W. J. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry. 1979 Nov 27;18(24):5294–5299. doi: 10.1021/bi00591a005. [DOI] [PubMed] [Google Scholar]
  3. Chourey P. S., Nelson O. E. The enzymatic deficiency conditioned by the shrunken-1 mutations in maize. Biochem Genet. 1976 Dec;14(11-12):1041–1055. doi: 10.1007/BF00485135. [DOI] [PubMed] [Google Scholar]
  4. Echt C. S., Chourey P. S. A Comparison of Two Sucrose Synthetase Isozymes from Normal and shrunken-1 Maize. Plant Physiol. 1985 Oct;79(2):530–536. doi: 10.1104/pp.79.2.530. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hake S., Kelley P. M., Taylor W. C., Freeling M. Coordinate induction of alcohol dehydrogenase 1, aldolase, and other anaerobic RNAs in maize. J Biol Chem. 1985 Apr 25;260(8):5050–5054. [PubMed] [Google Scholar]
  6. Hentze M. W., Caughman S. W., Rouault T. A., Barriocanal J. G., Dancis A., Harford J. B., Klausner R. D. Identification of the iron-responsive element for the translational regulation of human ferritin mRNA. Science. 1987 Dec 11;238(4833):1570–1573. doi: 10.1126/science.3685996. [DOI] [PubMed] [Google Scholar]
  7. Hentze M. W., Rouault T. A., Caughman S. W., Dancis A., Harford J. B., Klausner R. D. A cis-acting element is necessary and sufficient for translational regulation of human ferritin expression in response to iron. Proc Natl Acad Sci U S A. 1987 Oct;84(19):6730–6734. doi: 10.1073/pnas.84.19.6730. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Jackson A. O., Larkins B. A. Influence of Ionic Strength, pH, and Chelation of Divalent Metals on Isolation of Polyribosomes from Tobacco Leaves. Plant Physiol. 1976 Jan;57(1):5–10. doi: 10.1104/pp.57.1.5. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kelley P. M., Freeling M. Anaerobic expression of maize fructose-1,6-diphosphate aldolase. J Biol Chem. 1984 Nov 25;259(22):14180–14183. [PubMed] [Google Scholar]
  10. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  11. McCarty D. R., Shaw J. R., Hannah L. C. The cloning, genetic mapping, and expression of the constitutive sucrose synthase locus of maize. Proc Natl Acad Sci U S A. 1986 Dec;83(23):9099–9103. doi: 10.1073/pnas.83.23.9099. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. McElfresh K. C., Chourey P. S. Anaerobiosis induces transcription but not translation of sucrose synthase in maize. Plant Physiol. 1988 Jun;87(2):542–546. doi: 10.1104/pp.87.2.542. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Rogers J., Munro H. Translation of ferritin light and heavy subunit mRNAs is regulated by intracellular chelatable iron levels in rat hepatoma cells. Proc Natl Acad Sci U S A. 1987 Apr;84(8):2277–2281. doi: 10.1073/pnas.84.8.2277. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Ryazanov A. G., Shestakova E. A., Natapov P. G. Phosphorylation of elongation factor 2 by EF-2 kinase affects rate of translation. Nature. 1988 Jul 14;334(6178):170–173. doi: 10.1038/334170a0. [DOI] [PubMed] [Google Scholar]
  15. Springer B., Werr W., Starlinger P., Bennett D. C., Zokolica M., Freeling M. The Shrunken gene on chromosome 9 of Zea mays L is expressed in various plant tissues and encodes an anaerobic protein. Mol Gen Genet. 1986 Dec;205(3):461–468. doi: 10.1007/BF00338083. [DOI] [PubMed] [Google Scholar]
  16. Su J. C., Preiss J. Purification and properties of sucrose synthase from maize kernels. Plant Physiol. 1978 Mar;61(3):389–393. doi: 10.1104/pp.61.3.389. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Thomas P. S. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201–5205. doi: 10.1073/pnas.77.9.5201. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Werr W., Frommer W. B., Maas C., Starlinger P. Structure of the sucrose synthase gene on chromosome 9 of Zea mays L. EMBO J. 1985 Jun;4(6):1373–1380. doi: 10.1002/j.1460-2075.1985.tb03789.x. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES