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. 1989 Aug;90(4):1422–1428. doi: 10.1104/pp.90.4.1422

Separation and Characterization of Inositol Phospholipids from the Pulvini of Samanea saman1

Gary G Coté 1, Anthony L DePass 1,2, Lynne M Quarmby 1, Bonnie F Tate 1, M J Morse 1,3, Ruth L Satter 1, Richard C Crain 1
PMCID: PMC1061906  PMID: 16666946

Abstract

To supplement current thin-layer chromatographic methods for separation and quantitation of plant phospholipids, an alternative method, high-performance liquid chromatography was developed. The major inositol-containing lipids from the pulvini of Samanea saman Merr. were identified as phosphatidylinositol, phosphatidylinositol phosphate, and phosphatidylinositol bisphosphate based on comigration with authentic standards on high-performance liquid chromatography and on thin-layer chromatography. The patterns of incorporation of radioactivity into the putative phosphatidylinositol and phosphatidylinositol phosphate were consistent with these identifications when pulvini were labeled with [3H]glycerol, [3H]inositol, or [32P]orthophosphate. Analysis of the products of enzymic hydrolysis, of chemical deacylation, and of `fingerprint' methanolysis of these phospholipids confirmed the identifications.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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