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. 1989 Aug;90(4):1429–1434. doi: 10.1104/pp.90.4.1429

Identification and Characterization of the Ca2+-ATPase which Drives Active Transport of Ca2+ at the Plasma Membrane of Radish Seedlings

Franca Rasi-Caldogno 1,2, Maria Chiara Pugliarello 1,2, Claudio Olivari 1,2, Maria Ida De Michelis 1,2
PMCID: PMC1061907  PMID: 16666947

Abstract

In microsomes from 24-hour-old radish (Raphanus sativus L.) seedlings ATP-dependent Ca2+ uptake occurs only in inside-out plasma membrane vesicles (F Rasi-Caldogno, MC Pugliarello, MI De Michelis [1987] Plant Physiol 83: 994-1000). A Ca2+-dependent ATPase activity can be shown in the same microsomes, when assays are performed at pH 7.5. The Ca2+-dependent ATPase is stimulated by the Ca2+ ionophore A23187 and is localized at the plasma membrane. Ca2+-dependent ATPase activity and ATP-dependent Ca2+ uptake present very similar saturation kinetics with erythrosin B (50% inhibition at about 0.1 micromolar), free Ca2+ (half-maximal rate at about 70 nanomolar), and MgATP (Km 15-20 micromolar). Ca2+ uptake can be sustained by GTP or ITP at about 60% the rate measured in the presence of ATP; only very low Ca2+ uptake is sustained by CTP or UTP and none by ADP. These results indicate that the Ca2+-ATPase described in this paper is the enzyme which drives active transport of Ca2+ at the plasma membrane of higher plants.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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