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. 1989 Aug;90(4):1538–1545. doi: 10.1104/pp.90.4.1538

Transfer of C4 Photosynthetic Characters through Hybridization of Flaveria Species 1

Randall G Cameron 1,2,3, Carole L Bassett 1,2,3, Joe H Bouton 1,2,3, R Harold Brown 1,2,3
PMCID: PMC1061922  PMID: 16666962

Abstract

Transfer of C4 photosynthetic traits was studied through hybridization of Flaveria trinervia (Spreng.) Mohr (C4) and Flaveria brownii A.M. Powell (C4-like) with Flaveria linearis Lag. (C3-C4) and the C3 species Flaveria pringlei Gandoger (C3). Fertility was low, based on irregular chromosome pairing and low pollen stainability, except in F. brownii × F. linearis which had bivalent pairing and 76% stainable pollen. Hybrids had apparent photosynthesis values of 71 to 148% of the midparental means, while the CO2 compensation concentration was similar to the C4 or C4-like parent, except in hybrids having the C3 species F. pringlei as a parent. Inhibition of apparent photosynthesis by O2, and phosphoenolpyruvate carboxylase and NADP-malic enzyme activities and subunit levels in the hybrids were closer to the C3 or C3-C4 parent. The species F. brownii and F. trinervia were equal in their capacity to transfer reduced O2 inhibition of AP and CO2 compensation concentration values to hybrids with F. linearis (C3-C4), although hybrids with F. trinervia had higher PEPC activity. The O2 inhibition of AP was correlated with the logarithm of activities of phosphoenolpyruvate carboxylase (r = −0.95) and NADP-malic enzyme (r = −0.87). These results confirm that C4 traits can be transferred by hybridization of C3-C4 and C4 or C4-like species, with a higher degree of C4 photosynthesis than exists in C3-C4 species, and at least in F. brownii × F. linearis, fertile progeny are obtained.

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Selected References

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