Abstract
The variations of the amounts of phospholipid transfer proteins (PLTP), determined by ELISA and immunoblotting methods, were followed during the maturation and germination of maize (Zea mays L.) seeds. Changes of the amounts of PLTP occur during seed maturation. The levels of PLTP, low in the first 3 weeks after fecondation, strongly raised 3 to 5 weeks after, then reached and maintained a high value (10% of total soluble proteins) during the last steps of maturation. These variations, determined by ELISA, are in accordance with the observations made by immunoblotting. Changes in phospholipid transfer activity were also found when protein extracts prepared from seeds at different stages of maturation were assayed for transfer activity. The levels of PLTP were also determined during the germination of maize seeds and the early growth of the plantlets, both in the endosperm and the aerial parts. While no major change was observed in the endosperm, a high increase in PLTP level was found in the aerial part of the plantlet, both by ELISA and immunoblotting. An enhancement of the phospholipid transfer activity was parallely observed in the protein extracts of plantlets at various stages of germination. These results are consistent with an in vivo correlation between the synthesis of phospholipid transfer protein, observed during the maturation and germination of maize seeds, and the biogenesis of membranes which involves intracellular movements of phospholipids.
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- Brophy P. J., Aitken J. W. Phosphatidylinositol transfer activity in rat cerebral hemispheres during development. J Neurochem. 1979 Jul;33(1):355–356. doi: 10.1111/j.1471-4159.1979.tb11741.x. [DOI] [PubMed] [Google Scholar]
- Engle M. J., van Golde L. M., Wirtz K. W. Transfer of phospholipids between subcellular fractions of the lung. FEBS Lett. 1978 Feb 15;86(2):277–281. doi: 10.1016/0014-5793(78)80579-1. [DOI] [PubMed] [Google Scholar]
- Hannapel D. J., Ohlrogge J. B. Regulation of Acyl Carrier Protein Messenger RNA Levels during Seed and Leaf Development. Plant Physiol. 1988 Apr;86(4):1174–1178. doi: 10.1104/pp.86.4.1174. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kader J. C., Julienne M., Vergnolle C. Purification and characterization of a spinach-leaf protein capable of transferring phospholipids from liposomes to mitochondria or chloroplasts. Eur J Biochem. 1984 Mar 1;139(2):411–416. doi: 10.1111/j.1432-1033.1984.tb08020.x. [DOI] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- Ohlrogge J. B., Kuo T. M. Control of Lipid Synthesis during Soybean Seed Development: Enzymic and Immunochemical Assay of Acyl Carrier Protein. Plant Physiol. 1984 Mar;74(3):622–625. doi: 10.1104/pp.74.3.622. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tchang F., Laroche-Raynal M., Vergnolle C., Demandre C., Douady D., Grosbois M., Guerbette F., Delseny M., Kader J. C. In vitro synthesis of a plant phospholipid transfer protein: a study by high performance liquid chromatography. Biochem Biophys Res Commun. 1985 Nov 27;133(1):75–81. doi: 10.1016/0006-291x(85)91843-1. [DOI] [PubMed] [Google Scholar]
- Tchang F., This P., Stiefel V., Arondel V., Morch M. D., Pages M., Puigdomenech P., Grellet F., Delseny M., Bouillon P. Phospholipid transfer protein: full-length cDNA and amino acid sequence in maize. Amino acid sequence homologies between plant phospholipid transfer proteins. J Biol Chem. 1988 Nov 15;263(32):16849–16855. [PubMed] [Google Scholar]
- Van der Krift T. P., Leunissen J., Teerlink T., Van Heusden G. P., Verkleij A. J., Wirtz K. W. Ultrastructural localization of a peroxisomal protein in rat liver using the specific antibody against the non-specific lipid transfer protein (sterol carrier protein 2). Biochim Biophys Acta. 1985 Jan 25;812(2):387–392. doi: 10.1016/0005-2736(85)90313-x. [DOI] [PubMed] [Google Scholar]
- Vergnolle C., Arondel V., Tchang F., Grosbois M., Guerbette F., Jolliot A., Kader J. C. Synthesis of phospholipid transfer proteins from maize seedlings. Biochem Biophys Res Commun. 1988 Nov 30;157(1):37–41. doi: 10.1016/s0006-291x(88)80007-x. [DOI] [PubMed] [Google Scholar]