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. 1989 Sep;91(1):401–408. doi: 10.1104/pp.91.1.401

Enhanced Thermal Tolerance in a Mutant of Arabidopsis Deficient in Palmitic Acid Unsaturation 1

Ljerka Kunst 1,2, John Browse 1,2, Chris Somerville 1,2
PMCID: PMC1062006  PMID: 16667033

Abstract

A mutant of Arabidopsis thaliana, deficient in the activity of a chloroplast ω9 fatty acid desaturase, accumulates high amounts of palmitic acid (16:0), and exhibits an overall reduction in the level of unsaturation of chloroplast lipids. Under standard conditions the altered membrane lipid composition had only minor effects on growth rate of the mutant, net photosynthetic CO2 fixation, photosynthetic electron transport, or chloroplast ultrastructure. Similarly, fluorescence polarization measurements indicated that the fluidity of the membranes was not significantly different in the mutant and the wild type. However, at temperatures above 28°C, the mutant grew more rapidly than the wild type suggesting that the altered fatty acid composition enhanced the thermal tolerance of the mutant. Similarly, the chloroplast membranes of the mutant were more resistant than wild type to thermal inactivation of photosynthetic electron transport. These observations lend support to previous suggestions that chloroplast membrane lipid composition may be an important component of the thermal acclimation response observed in many plant species which are photosynthetically active during periods of seasonally variable temperature extremes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Andersson B., Anderson J. M., Ryrie I. J. Transbilayer organization of the chlorophyll-proteins of spinach thylakoids. Eur J Biochem. 1982 Apr 1;123(2):465–472. doi: 10.1111/j.1432-1033.1982.tb19790.x. [DOI] [PubMed] [Google Scholar]
  2. Browse J., Kunst L., Anderson S., Hugly S., Somerville C. A mutant of Arabidopsis deficient in the chloroplast 16:1/18:1 desaturase. Plant Physiol. 1989 Jun;90(2):522–529. doi: 10.1104/pp.90.2.522. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Downton W. J., Berry J. A., Seemann J. R. Tolerance of photosynthesis to high temperature in desert plants. Plant Physiol. 1984 Apr;74(4):786–790. doi: 10.1104/pp.74.4.786. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hugly S., Kunst L., Browse J., Somerville C. Enhanced thermal tolerance of photosynthesis and altered chloroplast ultrastructure in a mutant of Arabidopsis deficient in lipid desaturation. Plant Physiol. 1989 Jul;90(3):1134–1142. doi: 10.1104/pp.90.3.1134. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Kunst L., Browse J., Somerville C. A mutant of Arabidopsis deficient in desaturation of palmitic Acid in leaf lipids. Plant Physiol. 1989 Jul;90(3):943–947. doi: 10.1104/pp.90.3.943. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kunst L., Browse J., Somerville C. Altered chloroplast structure and function in a mutant of Arabidopsis deficient in plastid glycerol-3-phosphate acyltransferase activity. Plant Physiol. 1989 Jul;90(3):846–853. doi: 10.1104/pp.90.3.846. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Leech R. M., Rumsby M. G., Thomson W. W. Plastid differentiation, acyl lipid, and Fatty Acid changes in developing green maize leaves. Plant Physiol. 1973 Sep;52(3):240–245. doi: 10.1104/pp.52.3.240. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Lynch D. V., Thompson G. A. Chloroplast Phospholipid Molecular Species Alterations during Low Temperature Acclimation in Dunaliella. Plant Physiol. 1984 Feb;74(2):198–203. doi: 10.1104/pp.74.2.198. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Markwell J. P., Thornber J. P., Boggs R. T. Higher plant chloroplasts: Evidence that all the chlorophyll exists as chlorophyll-protein complexes. Proc Natl Acad Sci U S A. 1979 Mar;76(3):1233–1235. doi: 10.1073/pnas.76.3.1233. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. McCourt P., Browse J., Watson J., Arntzen C. J., Somerville C. R. Analysis of Photosynthetic Antenna Function in a Mutant of Arabidopsis thaliana (L.) Lacking trans-Hexadecenoic Acid. Plant Physiol. 1985 Aug;78(4):853–858. doi: 10.1104/pp.78.4.853. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. McCourt P., Kunst L., Browse J., Somerville C. R. The effects of reduced amounts of lipid unsaturation on chloroplast ultrastructure and photosynthesis in a mutant of Arabidopsis. Plant Physiol. 1987 Jun;84(2):353–360. doi: 10.1104/pp.84.2.353. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Pearcy R. W. Effect of Growth Temperature on the Fatty Acid Composition of the Leaf Lipids in Atriplex lentiformis (Torr.) Wats. Plant Physiol. 1978 Apr;61(4):484–486. doi: 10.1104/pp.61.4.484. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Rawyler A., Siegenthaler P. A. Transmembrane distribution of phospholipids and their involvement in electron transport, as revealed by phospholipids A2 treatment of spinach thylakoids. Biochim Biophys Acta. 1981 Apr 13;635(2):348–358. doi: 10.1016/0005-2728(81)90033-5. [DOI] [PubMed] [Google Scholar]
  14. Stubbs C. D., Smith A. D. The modification of mammalian membrane polyunsaturated fatty acid composition in relation to membrane fluidity and function. Biochim Biophys Acta. 1984 Jan 27;779(1):89–137. doi: 10.1016/0304-4157(84)90005-4. [DOI] [PubMed] [Google Scholar]
  15. Vigh L., Joó F., Droppa M., Horváth L. I., Horváth G. Modulation of chloroplast membrane lipids by homogeneous catalytic hydrogenation. Eur J Biochem. 1985 Mar 15;147(3):477–481. doi: 10.1111/j.0014-2956.1985.00477.x. [DOI] [PubMed] [Google Scholar]

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