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. 1989 Oct;91(2):451–454. doi: 10.1104/pp.91.2.451

DNA Methylation is Reduced in DNasel-Sensitive Regions of Plant Chromatin 1

Manfred Klaas 1,2, Richard M Amasino 1,3
PMCID: PMC1062017  PMID: 16667051

Abstract

Pea, barley, and corn chromatin was isolated and subjected to limited digestion with DNasel. The preferentially degraded, small molecular weight fraction, presumably consisting of sequences in an active state of expression, was isolated and its 5-methylcytosine content determined. The DNasel-sensitive chromatin fraction from all three plant species investigated contained a markedly reduced level of DNA methylation compared to total DNA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Amasino R. M., Powell A. L., Gordon M. P. Changes in T-DNA methylation and expression are associated with phenotypic variation and plant regeneration in a crown gall tumor line. Mol Gen Genet. 1984;197(3):437–446. doi: 10.1007/BF00329940. [DOI] [PubMed] [Google Scholar]
  2. Antequera F., Bird A. P. Unmethylated CpG islands associated with genes in higher plant DNA. EMBO J. 1988 Aug;7(8):2295–2299. doi: 10.1002/j.1460-2075.1988.tb03072.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Ball D. J., Gross D. S., Garrard W. T. 5-methylcytosine is localized in nucleosomes that contain histone H1. Proc Natl Acad Sci U S A. 1983 Sep;80(18):5490–5494. doi: 10.1073/pnas.80.18.5490. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Becker P. B., Ruppert S., Schütz G. Genomic footprinting reveals cell type-specific DNA binding of ubiquitous factors. Cell. 1987 Nov 6;51(3):435–443. doi: 10.1016/0092-8674(87)90639-8. [DOI] [PubMed] [Google Scholar]
  5. Chandler V. L., Walbot V. DNA modification of a maize transposable element correlates with loss of activity. Proc Natl Acad Sci U S A. 1986 Mar;83(6):1767–1771. doi: 10.1073/pnas.83.6.1767. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Davie J. R., Saunders C. A. Chemical composition of nucleosomes among domains of calf thymus chromatin differing in micrococcal nuclease accessibility and solubility properties. J Biol Chem. 1981 Dec 10;256(23):12574–12580. [PubMed] [Google Scholar]
  7. Gelvin S. B., Karcher S. J., DiRita V. J. Methylation of the T-DNA in Agrobacterium tumefaciens and in several crown gall tumors. Nucleic Acids Res. 1983 Jan 11;11(1):159–174. doi: 10.1093/nar/11.1.159. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gruenbaum Y., Cedar H., Razin A. Substrate and sequence specificity of a eukaryotic DNA methylase. Nature. 1982 Feb 18;295(5850):620–622. doi: 10.1038/295620a0. [DOI] [PubMed] [Google Scholar]
  9. Hansen R. S., Ellis N. A., Gartler S. M. Demethylation of specific sites in the 5' region of the inactive X-linked human phosphoglycerate kinase gene correlates with the appearance of nuclease sensitivity and gene expression. Mol Cell Biol. 1988 Nov;8(11):4692–4699. doi: 10.1128/mcb.8.11.4692. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hepburn A. G., Clarke L. E., Pearson L., White J. The role of cytosine methylation in the control of nopaline synthase gene expression in a plant tumor. J Mol Appl Genet. 1983;2(3):315–329. [PubMed] [Google Scholar]
  11. Hoopes B. C., McClure W. R. Studies on the selectivity of DNA precipitation by spermine. Nucleic Acids Res. 1981 Oct 24;9(20):5493–5504. doi: 10.1093/nar/9.20.5493. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kamalay J. C., Goldberg R. B. Organ-specific nuclear RNAs in tobacco. Proc Natl Acad Sci U S A. 1984 May;81(9):2801–2805. doi: 10.1073/pnas.81.9.2801. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Miller O. J., Schnedl W., Allen J., Erlanger B. F. 5-Methylcytosine localised in mammalian constitutive heterochromatin. Nature. 1974 Oct 18;251(5476):636–637. doi: 10.1038/251636a0. [DOI] [PubMed] [Google Scholar]
  14. Naveh-Many T., Cedar H. Active gene sequences are undermethylated. Proc Natl Acad Sci U S A. 1981 Jul;78(7):4246–4250. doi: 10.1073/pnas.78.7.4246. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Reid R. A., John M. C., Amasino R. M. Deoxyribonuclease I sensitivity of the T-DNA ipt gene is associated with gene expression. Biochemistry. 1988 Jul 26;27(15):5748–5754. doi: 10.1021/bi00415a053. [DOI] [PubMed] [Google Scholar]
  16. Spiker S., Murray M. G., Thompson W. F. DNase I sensitivity of transcriptionally active genes in intact nuclei and isolated chromatin of plants. Proc Natl Acad Sci U S A. 1983 Feb;80(3):815–819. doi: 10.1073/pnas.80.3.815. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Walling L., Drews G. N., Goldberg R. B. Transcriptional and post-transcriptional regulation of soybean seed protein mRNA levels. Proc Natl Acad Sci U S A. 1986 Apr;83(7):2123–2127. doi: 10.1073/pnas.83.7.2123. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Watson J. C., Kaufman L. S., Thompson W. F. Developmental regulation of cytosine methylation in the nuclear ribosomal RNA genes of Pisum sativum. J Mol Biol. 1987 Jan 5;193(1):15–26. doi: 10.1016/0022-2836(87)90622-x. [DOI] [PubMed] [Google Scholar]
  19. Weisbrod S. Active chromatin. Nature. 1982 May 27;297(5864):289–295. doi: 10.1038/297289a0. [DOI] [PubMed] [Google Scholar]
  20. Weisbrod S., Weintraub H. Isolation of a subclass of nuclear proteins responsible for conferring a DNase I-sensitive structure on globin chromatin. Proc Natl Acad Sci U S A. 1979 Feb;76(2):630–634. doi: 10.1073/pnas.76.2.630. [DOI] [PMC free article] [PubMed] [Google Scholar]

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