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Comparisons of oxidative stress and senescence markers between mature sickle red blood cells containing mitochondria and mature sickle red blood cells without. (A) Ca2+ levels, (B) intracellular reactive oxygen species (ROS), (C) red blood cells (RBC) exposing phosphatidylserine (PS) and (D) CD47 expression. MFI: mean fluorescence intensity. mito: mitochondria. Significant difference: *P<0.05; **P<0.01; ****P<0.0001.
