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. 1989 Dec;91(4):1259–1261. doi: 10.1104/pp.91.4.1259

Antibodies against the Calcium-Binding Protein

Calsequestrin from Streptanthus tortuosus (Brassicaceae)

Mei Chou 1,2,1, Karl-Heinz Krause 1,2,2, Kevin P Campbell 1,2,3, Kenneth G Jensen 1,2, Richard D Sjolund 1,2,1
PMCID: PMC1062175  PMID: 16667172

Abstract

Plant microsomes contain a protein clearly related to a calcium-binding protein, calsequestrin, originally found in the sarcoplasmic reticulum of muscle cells, responsible for the rapid release and uptake of Ca2+ within the cells. The location and role of calsequestrin in plant cells is unknown. To generate monoclonal antibodies specific to plant calsequestrin, mice were immunized with a microsomal fraction from cultured cells of Streptanthus tortuosus (Brassicaceae). Two clones cross-reacted with one protein band with a molecular weight equal to that of calsequestrin (57 kilodaltons) by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotting. This band is able to bind 45Ca2+ and can be recognized by a polyclonal antibody against the canine cardiac muscle calsequestrin. Rabbit skeletal muscle calsequestrin cross-reacted with the plant monoclonal antibodies. The plant monoclonal antibodies generated here are specific to calsequestrin protein.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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