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. 1989 Dec;91(4):1476–1480. doi: 10.1104/pp.91.4.1476

Intracellular Localization of Enzymes of Fatty Acid-β-Oxidation in the Alga Cyanidium caldarium1

Wolfgang Gross 1,2
PMCID: PMC1062209  PMID: 16667204

Abstract

The intracellular distribution of enzymes, participating in the β-oxidation of fatty acids in the eucaryotic alga Cyanidium has been studied. After separating the organelles from a crude homogenate on a linear flotation gradient, the enzymes enoyl-CoA hydratase, hydroxyacyl-CoA dehydrogenase, and thiolase were present in the mitochondrial fraction (density: 1.19 gram per cubic centimeter). Activity of an acyl-CoA synthetase was found in the mitochondrial fraction as well as in a band where mitochondrial membrane apparently had accumulated (density: 1.17 gram per cubic centimeter). None of these enzymes were present in the peroxisomes (density: 1.23 gram per cubic centimeter). Results from cell fractionation as well as properties of β-oxidation enzymes indicate a mitochondrial location of fatty acid degradation also in the algae Galdieria sulphuraria and Cyanidioschyzon merolae.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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